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Literature summary for 3.4.22.28 extracted from

  • Bablanian, G.M.; Grubman, M.J.
    Characterization of the foot-and-mouth disease virus 3C protease expressed in Escherichia coli (1993), Virology, 197, 320-327.
    View publication on PubMed
Search for more literature for 3.4.22.28

Cloned(Commentary)

Cloned (Comment) Organism
cloned into a plasmid under the control of the bacteriophage T7 promoter, expressed both in an in vito transcription-translation system and in vivo in an Escherichia coli system containig an inducible T7 RNA polymerase Foot-and-mouth disease virus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane recombinant enzyme Foot-and-mouth disease virus 16020
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soluble recombinant enzyme Foot-and-mouth disease virus
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Organism

Organism UniProt Comment Textmining
Foot-and-mouth disease virus
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
foot-and-mouth-disease virus P1 precursor polyprotein + H2O recombinant foot-and-mouth-disease virus enzyme, cleaves VP3-VP1 site, followed by VP0-VP3 and VP1-2A site (least efficiently), kinetic of P1 processing Foot-and-mouth disease virus mature structural foot-and-mouth-disease virus proteins VPO-VP3 + VP1-2A (enzyme from supernatant), VPO + VP3 + VP1 (pellet enzyme) ?
foot-and-mouth-disease virus P1-2A polyprotein + H2O recombinant foot-and-mouth-disease virus enzyme, cleaves VP3-VP1 site initially, followed by VP0-VP3 and VP1-2A site (least efficiently), kinetic of P1-processing Foot-and-mouth disease virus mature structural foot-and-mouth-disease virus proteins VPO-VP3 + VP1-2A (enzyme from supernatant), VPO + VP3 + VP1 (pellet enzyme) ?
foot-and-mouth-disease virus P2 precursor protein + H2O
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 Foot-and-mouth disease virus foot-and-mouth-disease virus protein 2B + foot-and-mouth-disease virus protein 2C + foot-and-mouth-disease virus protein 2A-2B
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 ?