Literature summary for 3.4.22.2 extracted from

Chu, M.H.; Liu, K.L.; Wu, H.Y.; Yeh, K.W.; Cheng, Y.S.
Crystal structure of tarocystatin-papain complex: implications for the inhibition property of group-2 phytocystatins (2011), Planta, 234, 243-254.

Crystallization (Commentary)

Crystallization (Comment)	Organism
tarocystatin-papain complex and C-terminal cystatin-like extension-papain complex, hanging drop vapor diffusion method, the tarocystatin-papain complex is crystallized from a drop containing 15% PEG monomethyl ether 2000, 0.05 M sodium acetate trihydrate, pH 4.6, 0.1 M ammonium sulfate against a reservoir of 30% PEG monomethyl ether 2000, 0.1 M sodium acetate trihydrate, pH 4.6, and 0.2 M ammonium sulfate. The complex of CtE-papain is crystallized from a drop containing 0.05 M HEPES, pH 7.5, and 35% (v/v) 2-methyl-2,4-pentanediol against a reservoir of 0.1 M HEPES, pH 7.5, and 70% (v/v) 2-methyl-2,4-pentanediol	Carica papaya

Crystallization (Comment)

Organism

tarocystatin-papain complex and C-terminal cystatin-like extension-papain complex, hanging drop vapor diffusion method, the tarocystatin-papain complex is crystallized from a drop containing 15% PEG monomethyl ether 2000, 0.05 M sodium acetate trihydrate, pH 4.6, 0.1 M ammonium sulfate against a reservoir of 30% PEG monomethyl ether 2000, 0.1 M sodium acetate trihydrate, pH 4.6, and 0.2 M ammonium sulfate. The complex of CtE-papain is crystallized from a drop containing 0.05 M HEPES, pH 7.5, and 35% (v/v) 2-methyl-2,4-pentanediol against a reservoir of 0.1 M HEPES, pH 7.5, and 70% (v/v) 2-methyl-2,4-pentanediol

Carica papaya

Inhibitors

Inhibitors	Comment	Organism	Structure
tarocystatin	the N-terminal cystatin domain (residues 1-98) of tarocystatin has inhibitory ability against papain	Carica papaya

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
tarocystatin + H2O	Carica papaya	the C-terminal cystatin-like extension of tarocystatin is easily digested by papain	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Carica papaya	P00784	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
latex	-	Carica papaya	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
tarocystatin + H2O	the C-terminal cystatin-like extension of tarocystatin is easily digested by papain	Carica papaya	?	-	?