Protein Variants | Comment | Organism |
---|---|---|
K174R | mutation introduced according to thermostable homologue ervatamin C, unstable | Carica papaya |
K174R/V32S | mutation introduced according to thermostable homologue ervatamin C, improvement of thermal stability | Carica papaya |
K174R/V32S/G36S | mutation introduced according to thermostable homologue ervatamin C, improvement of thermal stability | Carica papaya |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Carica papaya | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
121 | - |
mutant K174R/V32S, pH 6.5, 37°C | Carica papaya |
128 | - |
wild-type, pH 6.5, 37°C | Carica papaya |
130 | - |
mutant K174R/V32S/G36S, pH 6.5, 37°C | Carica papaya |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
azocasein + H2O | - |
Carica papaya | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
wild-type | Carica papaya |
60 | - |
mutant K174R/V32S | Carica papaya |
65 | - |
mutant K174R/V32S/G36S | Carica papaya |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
half-life of wild-type, mutant K174R/V32S and mutant K174R/V32S/G36S is 77, 114, and 171 min, respectively | Carica papaya |
65 | - |
half-life of wild-type, mutant K174R/V32S and mutant K174R/V32S/G36S is 35, 45, and 80 min, respectively | Carica papaya |