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Literature summary for 3.4.22.2 extracted from
- The preparation and catalytically active characterization of papain immobilized on magnetic composite microspheres (2004), Enzyme Microb. Technol., 35, 15-21.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| biotechnology | papain immobilization on magnetic composite microspheres at pH 8.0 and 30°C, quantification and kinetics, overview, the immobilized enzyme exhibits better environmentally adaptability and reusability than the soluble enzyme | Carica papaya |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics and thermodynamics of soluble and immobilized enzyme at pH 7.0 and 40°C, overview | Carica papaya |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Carica papaya | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | - |
Carica papaya | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 60 days, the immobilized enzyme shows 20% loss of activity, the soluble enzyme shows 50% loss of activity | Carica papaya |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| casein + H2O | - |
Carica papaya | ? | - |
? |  |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
soluble enzyme | Carica papaya |
| 80 | - |
immobilized enzyme | Carica papaya |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 90 | temperature profile of soluble and immobilized enzyme | Carica papaya |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
30 min, the immobilized enzyme is completely stable, while the soluble enzyme loses activity | Carica papaya |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
soluble enzyme | Carica papaya |
| 8 | - |
immobilized enzyme | Carica papaya |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | pH profile of soluble and immobilized enzyme | Carica papaya |
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Release 2023.1 (January 2023)
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