Literature summary for 3.4.22.2 extracted from

Gutierrez-Gonzalez, L.H.; Rojo-Dominguez, A.; Cabrera-Gonzalez, N.E.; Perez-Montfort, R.; Padilla-Zuniga, A.J.
Loosely packed papain prosegment displays inhibitory activity (2006), Arch. Biochem. Biophys., 446, 151-160.

Search for more literature for 3.4.22.2

Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	loosely packed papain prosegment displays inhibitory activity but can also function as activator for the mature enzyme, overview	Carica papaya

Cloned(Commentary)

Cloned (Comment)	Organism
DNA sequence determination and analysis of the proenzyme, expression of the isolated papain prosegment in Escherichia coli strain BL21(DE3) in the insoluble fraction	Carica papaya

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	loosely packed papain prosegment displays inhibitory activity but can also function as activator for the mature enzyme, overview	Carica papaya

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	thermodynamics of the thermal unfolding of the recombinant prosegment, overview, pH-jump kinetics of the prosegment, overview	Carica papaya

Organism

Organism	UniProt	Comment	Textmining
Carica papaya	P00784	enzyme precursor	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
proteolytic modification	the proenzyme is activated by cleavage of the prosegment	Carica papaya

Purification (Commentary)

Purification (Comment)	Organism
recombinant papain prosegment solubilized and refolded from Escherichia coli strain BL21(DE3) inclusion bodies, to homogeneity	Carica papaya

Renatured (Commentary)

Renatured (Comment)	Organism
recombinant papain prosegment from Escherichia coli strain BL21(DE3) inclusion bodies by 2% Triton X-100, 8 M guanidinium hydrochloride, and dialysis against 50 mM phosphoric acid/NaOH buffer, pH 7.0, and centrifugation at 45000 rpm for 1 h	Carica papaya

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	molecular recognition of mature enzyme and prosegment, overview	Carica papaya	?	-	?
Nalpha-benzoyl-DL-Arg-4-nitroanilide + H2O	-	Carica papaya	Nalpha-benzoyl-DL-Arg + 4-nitroaniline	-	?

Subunits

Subunits	Comment	Organism
More	the papain prosegment shows a high degree of conformational flexibility, proenzyme structure molecular modeling	Carica papaya

Synonyms

Synonyms	Comment	Organism
papaya proteinase 1	-	Carica papaya
PPI	-	Carica papaya

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
20	-	assay at	Carica papaya

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
additional information	-	thermal unfolding of the prosegment, in acid medium the enzyme unfolds in a globule-like conformation, irreversible without intermediate states, overview	Carica papaya

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
additional information	-	pH-jump kinetics of the prosegment at pH 8 to 3.4	Carica papaya
6.6	-	assay at	Carica papaya

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0000019	-	papain prosegment	recombinant prosegment, pH 7.0, 20°C, inhibition kinetics	Carica papaya

pI Value

Organism	Comment	pI Value Maximum	pI Value
Carica papaya	recombinnat prosegment, isoelectric focusing	-	4.4

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Release 2023.1 (January 2023)