Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.22.15 extracted from

  • Lee, A.R.; Bak, H.J.; Kim, N.Y.; Kim, M.S.; Go, H.J.; Han, J.W.; Ahn, S.J.; Park, N.G.; Chung, J.K.; Lee, H.H.
    Cloning, heterologous expression, and enzymatic characterization of cathepsin L from starfish (Asterina pectinifera) (2012), Biosci. Biotechnol. Biochem., 76, 2342-2346.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Patiria pectinifera

Inhibitors

Inhibitors Comment Organism Structure
antipain
-
Patiria pectinifera
chymostatin
-
Patiria pectinifera
Co2+ 58.88% residual activity at 5 mM Patiria pectinifera
E-64
-
Patiria pectinifera
Hg2+ 19.61% residual activity at 5 mM Patiria pectinifera
leupeptin
-
Patiria pectinifera
additional information not inhibited by N-ethylmaleimide, phenylmethylsulfonyl fluoride, antiprotinin, EDTA, EGTA, 1,10-phenanthroline, and pepstatin A Patiria pectinifera
SDS 6.53% residual activity at 0.05% (w/v) Patiria pectinifera

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61000
-
x * 61000, pro-enzyme fused to GST, SDS-PAGE Patiria pectinifera

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Gelatin + H2O Patiria pectinifera
-
?
-
?

Organism

Organism UniProt Comment Textmining
Patiria pectinifera I6QMK7 synonym Asterina pectinifera, starfisch
-

Purification (Commentary)

Purification (Comment) Organism
glutathione Sepharose column chromatography, and gel filtration Patiria pectinifera

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Patiria pectinifera
-
muscle
-
Patiria pectinifera
-
stomach
-
Patiria pectinifera
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-Gly-Gly-Arg-7-amido-4-methylcoumarin + H2O
-
Patiria pectinifera benzyloxycarbonyl-Gly-Gly-Arg + 7-amino-4-methylcoumarin
-
?
benzyloxycarbonyl-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O
-
Patiria pectinifera benzyloxycarbonyl-Gly-Pro-Arg + 7-amino-4-methylcoumarin
-
?
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O most efficient substrate Patiria pectinifera benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
?
benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin + H2O
-
Patiria pectinifera benzyloxycarbonyl-Val-Val-Arg + 7-amino-4-methylcoumarin
-
?
Gelatin + H2O
-
Patiria pectinifera ?
-
?
additional information the recombinant enzyme is inefficient in hydrolyzing azocasein, fibrinogen, and bovine serum albumin Patiria pectinifera ?
-
?

Subunits

Subunits Comment Organism
? x * 61000, pro-enzyme fused to GST, SDS-PAGE Patiria pectinifera

Synonyms

Synonyms Comment Organism
CtL
-
Patiria pectinifera

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 50 the enzyme is stable between 20 and 50°C, but activity drops above 60°C Patiria pectinifera

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Patiria pectinifera