Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Patiria pectinifera |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
antipain | - |
Patiria pectinifera | |
chymostatin | - |
Patiria pectinifera | |
Co2+ | 58.88% residual activity at 5 mM | Patiria pectinifera | |
E-64 | - |
Patiria pectinifera | |
Hg2+ | 19.61% residual activity at 5 mM | Patiria pectinifera | |
leupeptin | - |
Patiria pectinifera | |
additional information | not inhibited by N-ethylmaleimide, phenylmethylsulfonyl fluoride, antiprotinin, EDTA, EGTA, 1,10-phenanthroline, and pepstatin A | Patiria pectinifera | |
SDS | 6.53% residual activity at 0.05% (w/v) | Patiria pectinifera |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
61000 | - |
x * 61000, pro-enzyme fused to GST, SDS-PAGE | Patiria pectinifera |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Gelatin + H2O | Patiria pectinifera | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Patiria pectinifera | I6QMK7 | synonym Asterina pectinifera, starfisch | - |
Purification (Comment) | Organism |
---|---|
glutathione Sepharose column chromatography, and gel filtration | Patiria pectinifera |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Patiria pectinifera | - |
muscle | - |
Patiria pectinifera | - |
stomach | - |
Patiria pectinifera | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzyloxycarbonyl-Gly-Gly-Arg-7-amido-4-methylcoumarin + H2O | - |
Patiria pectinifera | benzyloxycarbonyl-Gly-Gly-Arg + 7-amino-4-methylcoumarin | - |
? | |
benzyloxycarbonyl-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O | - |
Patiria pectinifera | benzyloxycarbonyl-Gly-Pro-Arg + 7-amino-4-methylcoumarin | - |
? | |
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O | most efficient substrate | Patiria pectinifera | benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin | - |
? | |
benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin + H2O | - |
Patiria pectinifera | benzyloxycarbonyl-Val-Val-Arg + 7-amino-4-methylcoumarin | - |
? | |
Gelatin + H2O | - |
Patiria pectinifera | ? | - |
? | |
additional information | the recombinant enzyme is inefficient in hydrolyzing azocasein, fibrinogen, and bovine serum albumin | Patiria pectinifera | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 61000, pro-enzyme fused to GST, SDS-PAGE | Patiria pectinifera |
Synonyms | Comment | Organism |
---|---|---|
CtL | - |
Patiria pectinifera |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 50 | the enzyme is stable between 20 and 50°C, but activity drops above 60°C | Patiria pectinifera |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Patiria pectinifera |