Cloned (Comment) | Organism |
---|---|
- |
Toxoplasma gondii |
Crystallization (Comment) | Organism |
---|---|
homology modeling of the mature domain using human cathepsin L as a template. Enzyme contains a non-canonical aspartic acid at the base of the predicted active site S2 pocket, which limits substrate access | Toxoplasma gondii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
toxostatin | endogenous inhibitor, acts both on cathepsin L and cathepsin B in the nanomolar range | Toxoplasma gondii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
Benzyloxycarbonyl-Lys-Arg | pH 6.5 | Toxoplasma gondii | |
0.0157 | - |
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | pH 6.5 | Toxoplasma gondii | |
0.0176 | - |
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin | pH 6.5 | Toxoplasma gondii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | enzyme has a putative transmembrane domain, which is cleaved from the mature enzyme. No membrane localization is detected | Toxoplasma gondii | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24000 | - |
x * 32000, SDS-PAGE of partially matured protein, x * 24000, calculated for mautre protein | Toxoplasma gondii |
32000 | - |
x * 32000, SDS-PAGE of partially matured protein, x * 24000, calculated for mautre protein | Toxoplasma gondii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Toxoplasma gondii | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | full-length zymogen consists of a deduced 221 amino acid mature protein, 125 amino acid proregion, and a 75 amino acid pre-region | Toxoplasma gondii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
tachyzoite | primarily localized to its apical end, but does not co-localize with any apical organelles, including rhoptries, dense granules, or micronemes | Toxoplasma gondii | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O | - |
Toxoplasma gondii | ? | - |
? | |
benzyloxycarbonyl-Lys-Arg + H2O | - |
Toxoplasma gondii | ? | - |
? | |
benzyloxycarbonyl-Lys-Gln-Lys-Leu-Arg-7-amido-4-methylcoumarin + H2O | preferred substrate | Toxoplasma gondii | ? | - |
? | |
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O | - |
Toxoplasma gondii | ? | - |
? | |
additional information | leucine is preferred over phenylalanine in position P2 | Toxoplasma gondii | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 32000, SDS-PAGE of partially matured protein, x * 24000, calculated for mautre protein | Toxoplasma gondii |
Synonyms | Comment | Organism |
---|---|---|
CPL | - |
Toxoplasma gondii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Toxoplasma gondii |