Cloned (Comment) | Organism |
---|---|
mutants expressed in Pichia pastoris | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
A205L | mutant shows an effective cathepsin K-like preference for Leu and Pro | Homo sapiens |
L67Y/A205L | mutation induces a switch of the enzymatic specificity toward small selective inhibitors and peptidyl substrates | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
E-64 | - |
Homo sapiens | |
N-(4-biphenylacetyl)-S-methylcysteine-(D)-Arg-Phe-beta-phenethylamide | also called Cat L inhibitor 7, specific inhibitor | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0011 | - |
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | mutant enzyme L67Y/A205L | Homo sapiens | |
0.0013 | - |
benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin | wild type enzyme | Homo sapiens | |
0.0087 | - |
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | wild type enzyme | Homo sapiens | |
0.0098 | - |
benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin | mutant enzyme L67Y/A205L | Homo sapiens | |
0.014 | - |
benzyloxycarbonyl-Gly-Pro-Arg-7-amido-4-methylcoumarin | mutant enzyme L67Y/A205L | Homo sapiens | |
0.081 | - |
benzyloxycarbonyl-Gly-Pro-Arg-7-amido-4-methylcoumarin | wild type enzyme | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
butyl-Sepharose column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzyloxycarbonyl-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | benzyloxycarbonyl-Gly-Pro-Arg + 7-amino-4-methylcoumarin | - |
? | |
benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | benzyloxycarbonyl-Leu-Arg + 7-amino-4-methylcoumarin | - |
? | |
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin | - |
? | |
Collagen + H2O | substrate for cathepsin L mutant A205L | Homo sapiens | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
benzyloxycarbonyl-Gly-Pro-Arg-7-amido-4-methylcoumarin | wild type enzyme | Homo sapiens | |
0.5 | - |
benzyloxycarbonyl-Gly-Pro-Arg-7-amido-4-methylcoumarin | mutant enzyme L67Y/A205L | Homo sapiens | |
5.4 | - |
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | wild type enzyme | Homo sapiens | |
17.6 | - |
benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin | wild type enzyme | Homo sapiens | |
20.5 | - |
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | mutant enzyme L67Y/A205L | Homo sapiens | |
27.2 | - |
benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin | mutant enzyme L67Y/A205L | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0043 | - |
N-(4-biphenylacetyl)-S-methylcysteine-(D)-Arg-Phe-beta-phenethylamide | wild type enzyme | Homo sapiens |