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Literature summary for 3.4.22.15 extracted from

  • Fairhead, M.; van der Walle, C.F.
    The heavy-light chain loop of human cathepsin-L modulates its activity and stability (2008), Protein Pept. Lett., 15, 47-53.
    View publication on PubMed
Search for more literature for 3.4.22.15

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris strain X-33 Homo sapiens

Protein Variants

Protein Variants Comment Organism
T223V the mutation removes the potential glycosylation site and shows reduced enzyme activity Homo sapiens
T223V/DELTAE286-E289 the mutation shows reduced enzyme activity Homo sapiens
T223V/DELTAE286-N293 the mutation shows reduced enzyme activity Homo sapiens
T223V/M274L/D275N/G277A/V278M the mutation shows reduced enzyme activity Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
E-64
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0012
-
 benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin wild type enzyme Homo sapiens
0.0014
-
 benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin mutant enzyme T223V/DELTAE286-E289 Homo sapiens
0.0015
-
 benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin mutant enzyme T223V/M274L/D275N/G277A/V278M Homo sapiens
0.0017
-
 benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin mutant enzyme T223V/DELTAE286-N293 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
 recombinant enzyme, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P07711
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Phenyl-Sepharose column chromatography, and SP-Sepharose column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-casein + H2O
-
 Homo sapiens ?
-
 ?
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?

Synonyms

Synonyms Comment Organism
cathepsin-L
-
 Homo sapiens
cathepsin-L T2V
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
9
-
 benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin mutant enzyme T223V/M274L/D275N/G277A/V278M Homo sapiens
18
-
 benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin mutant enzyme T223V/DELTAE286-E289 Homo sapiens
30
-
 benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin mutant enzyme T223V/DELTAE286-N293 Homo sapiens
46
-
 benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin wild type enzyme Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 6
-
 Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
4 7
-
 Homo sapiens