Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris strain X-33 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
T223V | the mutation removes the potential glycosylation site | Homo sapiens |
T223V/C138S | the mutations prevent catalytic activity | Homo sapiens |
T223V/C138S/K99A/K104A | the mutations disrupt the putative heparin binding site | Homo sapiens |
General Stability | Organism |
---|---|
heparin reduces the stability of the pro form of cathepsin L at pH 5 by binding to a putative heparin binding motif (BBXB) in the pro-domain, in contrast heparin only slightly destabilizes the mature cathepsin L domain | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P07711 | procathepsin L | - |
Purification (Comment) | Organism |
---|---|
heparin-Sepharose chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
procathepsin L + H2O | autoactivation | Homo sapiens | cathepsin L + ? | - |
? |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | - |
the mature form of cathepsin L exhibits very little activity at pH 8.0 and is largely unfolded | Homo sapiens |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Homo sapiens | calculated value from mature cathepsin L | - |
4.7 |
Homo sapiens | calculated value from procathepsin L | - |
5.3 |