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Literature summary for 3.4.22.15 extracted from

  • Kirschke, H.; Kembhavi, A.A.; Bohley, P.; Barrett, A.J.
    Action of rat liver cathepsin L on collagen and other substrates (1982), Biochem. J., 201, 376-372.
No PubMed abstract available
Search for more literature for 3.4.22.15

General Stability

General Stability Organism
very susceptible to loss of activity through autolysis Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
Hg2+
-
 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
-
 Rattus norvegicus
0.01
-
 benzyloxycarbonyl-Lys-p-nitrophenyl ester
-
 Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
azocasein + H2O
-
 Rattus norvegicus ?
-
 ?
benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide + H2O weak activity Rattus norvegicus ?
-
 ?
benzyloxycarbonyl-Lys-p-nitrophenyl ester + H2O
-
 Rattus norvegicus benzyloxycarbonyl-Lys + 4-nitrophenol
-
 ?
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide + H2O
-
 Rattus norvegicus ?
-
 ?
Collagen + H2O selective cleavage of terminal peptides leads to conversion of beta- and higher components mainly to alpha-chains Rattus norvegicus ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
19.8
-
 benzyloxycarbonyl-Lys-p-nitrophenyl ester
-
 Rattus norvegicus
26
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
-
 Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
3.5 6 hydrolysis of collagen is 5fold faster at pH 3.5 than at pH 6.0 Rattus norvegicus