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Literature summary for 3.4.22.15 extracted from
- The purification and properties of cathepsin L from rabbit liver (1984), Biochem. J., 217, 209-217.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| benzyloxycarbonyl-Phe-Ala-CNH2 | - |
Oryctolagus cuniculus |  |
| Benzyloxycarbonyl-Phe-Phe-CHN2 | - |
Oryctolagus cuniculus | |
| Ep-460 | - |
Oryctolagus cuniculus | |
| Ep-475 | - |
Oryctolagus cuniculus | |
| Hg2+ | HgCl2; weak | Oryctolagus cuniculus | |
| iodoacetic acid | - |
Oryctolagus cuniculus | |
| leupeptin | - |
Oryctolagus cuniculus | |
| tosyl-Lys-CH2Cl | - |
Oryctolagus cuniculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0007 | - |
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide | - |
Oryctolagus cuniculus | |
| 0.005 | - |
succinyl-Ala-Phe-Lys-4-methylcoumarin 7-amide | - |
Oryctolagus cuniculus | |
| 0.006 | - |
benzoyl-Phe-Val-Arg-4-methylcoumarin 7-amide | - |
Oryctolagus cuniculus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29000 | - |
gel filtration | Oryctolagus cuniculus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryctolagus cuniculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Oryctolagus cuniculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Oryctolagus cuniculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 34.4 | - |
- |
Oryctolagus cuniculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-carboxypropionyl-Ala-Phe-Lys-4-methylcoumarin 7-amide + H2O | - |
Oryctolagus cuniculus | ? | - |
? | |
| Arg-4-methylcoumarin 7-amide + H2O | - |
Oryctolagus cuniculus | Arg + 7-amino-4-methylcoumarin | - |
? | |
| azocasein + H2O | - |
Oryctolagus cuniculus | ? | - |
? | |
| benzoyl-Phe-Val-Arg-4-methylcoumarin 7-amide + H2O | - |
Oryctolagus cuniculus | ? | - |
? | |
| benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide + H2O | weak activity | Oryctolagus cuniculus | ? | - |
? | |
| benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide + H2O | - |
Oryctolagus cuniculus | ? | - |
? | |
| Collagen + H2O | - |
Oryctolagus cuniculus | ? | - |
? | |
| succinyl-Ala-Phe-Lys-4-methylcoumarin 7-amide + H2O | - |
Oryctolagus cuniculus | ? | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 20 | - |
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide | - |
Oryctolagus cuniculus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.3 | - |
hydrolysis of collagen | Oryctolagus cuniculus |
| 5.2 | - |
hydrolysis of azocasein | Oryctolagus cuniculus |
| 6 | - |
hydrolysis of benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide | Oryctolagus cuniculus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.3 | 3.8 | about 60% of maximal activity at pH 3.0 and at pH 3.8, hydrolysis of collagen | Oryctolagus cuniculus |
| 4.5 | 6.5 | pH 4.5: about 75% of maximal activity, pH 6.5: about 60% of maximal activity, hydrolysis of azocasein | Oryctolagus cuniculus |
| 5.5 | 6.5 | pH 5.5: about 60% of maximal activity, pH 6.5: about 65% of maximal activity, hydrolysis of benzyloxycarbonyl-Phe-Arg-4-methycoumarin 7-amide | Oryctolagus cuniculus |
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Release 2023.1 (January 2023)
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