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Literature summary for 3.4.22.15 extracted from

  • McDonald, J.K.; Kadkhodayan, S.
    Cathepsin L: a latent proteinase in guinea pig sperm (1988), Biochem. Biophys. Res. Commun., 151, 827-835.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Urea enhances hydrolysis of azocasein Cavia porcellus

Inhibitors

Inhibitors Comment Organism Structure
Benzyloxycarbonyl-Phe-Phe-CHN2
-
Cavia porcellus
gossypol
-
Cavia porcellus
Hg2+
-
Cavia porcellus
iodoacetic acid
-
Cavia porcellus
L-trans-epoxysuccinyl-leucylamido(3-methyl)butane
-
Cavia porcellus
leupeptin
-
Cavia porcellus
Mersalyl acid
-
Cavia porcellus
tosyl-Lys-CH2Cl
-
Cavia porcellus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0011
-
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
-
Cavia porcellus

Organism

Organism UniProt Comment Textmining
Cavia porcellus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the fully-latent enzyme can be unmasked by incubating epididymal sperm for 2 h at pH 3.5 and 37°C Cavia porcellus

Source Tissue

Source Tissue Comment Organism Textmining
semen
-
Cavia porcellus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
azocasein + H2O
-
Cavia porcellus ?
-
?
benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide + H2O no activity Cavia porcellus ?
-
?
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide + H2O
-
Cavia porcellus ?
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
hydrolysis of benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide Cavia porcellus

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
-
Cavia porcellus