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Literature summary for 3.4.21.95 extracted from

  • Siigur, E.; Samel, M.; Tonismaegi, K.; Subbi, J.; Reintamm, T.; Siigur, J.
    Isolation, properties and N-terminal amino acid sequence of a factor V activator from Vipera lebetina (levantine viper) snake venom (1998), Biochim. Biophys. Acta, 1429, 239-248.
    View publication on PubMed
Search for more literature for 3.4.21.95

Inhibitors

Inhibitors Comment Organism Structure
diisopropylfluorophosphate
-
 Macrovipera lebetina

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28400
-
 gel filtration, MALDI-TOF mass spectrometry Macrovipera lebetina
30000
-
 1 * 30000, SDS-PAGE Macrovipera lebetina

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
factor V + H2O Macrovipera lebetina aside from factor V, no other protein substrate yet been identified ?
-
 ?

Organism

Organism UniProt Comment Textmining
Macrovipera lebetina
-
 Levantine viper
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Macrovipera lebetina

Purification (Commentary)

Purification (Comment) Organism
-
 Macrovipera lebetina

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2
-
 benzoylarginine ethyl ester as substrate Macrovipera lebetina
4.9
-
 Pro-Phe-Arg-4-methylcoumarin 7-amide as substrate Macrovipera lebetina

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Benzoylarginine ethyl ester + H2O
-
 Macrovipera lebetina ?
-
 ?
factor V + H2O
-
 Macrovipera lebetina ?
-
 ?
factor V + H2O aside from factor V, no other protein substrate yet been identified Macrovipera lebetina ?
-
 ?
additional information hydrolyzes several synthetic arginine ester substrates Macrovipera lebetina ?
-
 ?
Pro-Phe-Arg-4-methylcoumarin 7-amide + H2O
-
 Macrovipera lebetina ?
-
 ?
tosylarginine methyl ester + H2O
-
 Macrovipera lebetina ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 30000, SDS-PAGE Macrovipera lebetina

Synonyms

Synonyms Comment Organism
VLFVA
-
 Macrovipera lebetina

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 thermostable, heating for 20 min does not alter the arginine esterase activity of the enzyme Macrovipera lebetina

pI Value

Organism Comment pI Value Maximum pI Value
Macrovipera lebetina analytical isoelectric focusing studies reveals a protein band near the cathode, pI higer than 9.3
-
 9.3