Activating Compound | Comment | Organism | Structure |
---|---|---|---|
leptin | leptin stimulation of the prohormone convertase 1/3 promoter is regulated by Nhlh2 and STAT3 in vitro. Nhlh2 binds to both E-box motifs on the prohormone convertase 1/3 promoter. The Nhlh2 and STAT3 transcription factors heterodimerize and interact on the prohormone convertase 1/3 promoter. Leptin-treated N2KO mice have significantly higher prohormone convertase 1/3 mRNA levels than ad libitum-fed N2KO mice | Mus musculus |
Cloned (Comment) | Organism |
---|---|
into pGL3 basic vector to yield wild-type prohormone convertase 1/3 promoter construct, expressed in N29/2 cell line | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | PC1/3DELTA significantly decreases prohormone convertase 1/3 promoter activity by more than 60%. A 50% reduction when both E-boxes are mutated, even though the STAT3 sites remain intact (PC1/3DELTAE12). Mutation of both STAT3 sites (PC1/3DELTAS12) does not affect basal prohormone convertase 1/3 promoter activity, but leptin stimulation is lost. Mutating the E-box furthest from the start site (E-box 1, PC1/3DELTAE1) only has a significant effect on prohormone convertase 1/3 promoter activity under leptin stimulation, whereas mutating the E-box closest to the start site (E-box 2, PC1/3DELTAE2) has a more pronounced effect on luciferase expression with a loss of approximately 50% prohormone convertase 1/3 promoter activity levels in both leptin-stimulated and unstimulated cells | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | in wild-type mice prohormone convertase 1/3 mRNA levels show a 75% reduction with food deprivation and exceed ad libitum-fed levels after leptin treatment. Hypothalamic prohormone convertase 1/3 mRNA levels in N2KO mice show no significant variation between the deprived condition and ad libitum feeding. Leptin-treated N2KO mice have significantly lower prohormone convertase 1/3 mRNA levels than leptin-treated wild-type mice | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
hypothalamus | arcuate nucleus | Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | prohormone convertase 1/3 mRNA levels in the arcuate nucleus respond normally to signals of energy availability in wild-type mice but not in N2KO mice | Mus musculus | ? | - |
? | |
proopiomelanocortin + H2O | is cleaved by prohormone convertase 1/3 to produce peptides that regulate the body's response to energy availability | Mus musculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PC1/3 | - |
Mus musculus |
prohormone convertase 1/3 | - |
Mus musculus |