Literature summary for 3.4.21.93 extracted from

Lou, H.; Smith, A.M.; Coates, L.C.; Cawley, N.X.; Loh, Y.P.; Birch, N.P.
The transmembrane domain of the prohormone convertase PC3: a key motif for targeting to the regulated secretory pathway (2007), Mol. Cell. Endocrinol., 267, 17-25.

Search for more literature for 3.4.21.93

Application

Application	Comment	Organism
additional information	transmembrane domain of PC3 plays a key role in sorting the enzyme to the regulated secretory pathway	Rattus norvegicus

Cloned(Commentary)

Cloned (Comment)	Organism
full-length PC3, mutant PC3-deltaTM and truncated forms PC3 (1-638) and PC3 (1-616) expressed in Neuro2A cells	Rattus norvegicus

Protein Variants

Protein Variants	Comment	Organism
additional information	carboxyl terminus-truncated PC3 (1-638) containing the transmembrane domain is associated with lipid rafts in Neuro2A cells, while PC3 (1-616) and PC3-deltaTM lacking the transmembrane domain are not. PC3 (1-638) undergoes stimulated secretion and is colocalized with the secretory granule marker, chromogranin A, by immunocytochemistry. In contrast, PC3 (1-616) and PC3-deltaTM are constitutively secreted and primarily localized in the Golgi	Rattus norvegicus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	full length PC3 is associated with lipid rafts in Neuro2A cells, undergoes stimulated secretion and is colocalized with the secretory granule marker, chromogranin A, by immunocytochemistry	Rattus norvegicus	16020	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
87000	-	full-length PC3, Western blot analysis	Rattus norvegicus

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	-	-	-
Rattus norvegicus	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
AtT-20 cell	-	Mus musculus	-
Neuro-2a cell	very low levels of endogenous PC3	Mus musculus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Boc-Arg-Val-Arg-Arg-4-methyl-coumaryl-7-amide + H2O	-	Rattus norvegicus	?	-	?

Synonyms

Synonyms	Comment	Organism
prohormone convertase PC3	-	Mus musculus
prohormone convertase PC3	-	Rattus norvegicus

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Release 2023.1 (January 2023)