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Literature summary for 3.4.21.93 extracted from

  • Rabah, N.; Gauthier, D.J.; Gauthier, D.; Lazure, C.
    Improved PC1/3 production through recombinant expression in insect cells and larvae (2004), Protein Expr. Purif., 37, 377-384.
    View publication on PubMed
Search for more literature for 3.4.21.93

Cloned(Commentary)

Cloned (Comment) Organism
expression in Spodoptera frugiperda Sf9 cells and Trichoplusioa ni larvae using the baculovirus Autographa californica transfection system, method optimization, modification of the enzyme by C-terminal truncation and exchange of the PC1/3 signal peptide for the glycoprotein gp67 signal peptide, overview Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
PC1 pro-peptide inhibits the mature PC1 Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information the enzyme is localized in the regulated secretory pathway of neuroendocrine cells Mus musculus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
 Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
71000
-
 mature enzyme form, gel filtration Mus musculus
85000
-
 immature enzyme form, gel filtration Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Mus musculus the enzyme is involved in the regulated secretory pathway in neuroendocrine cells ?
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from insect cells and larvae, about 48fold from larvae by PEG precipitation, concanavalin A affinity and hydroxyapatite chromatography, and gel filtration Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
neuroendocrine cell regulated secretory pathway Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 different purified recombinant PC1 constructs, overview Mus musculus
44.45
-
 purified recombinant PC1 construct from Trichoplusia ni larvae Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme is involved in the regulated secretory pathway in neuroendocrine cells Mus musculus ?
-
 ?
pGlu-Arg-Thr-Lys-Arg-7-amido-4-methylcoumarin + H2O
-
 Mus musculus pGlu-Arg-Thr-Lys-Arg + 7-amino-4-methylcoumarin
-
 ?

Synonyms

Synonyms Comment Organism
PC1/3
-
 Mus musculus
protein convertase 1/3
-
 Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at room temperature Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 assay at Mus musculus