Literature summary for 3.4.21.73 extracted from

Ji, H.L.; Zhao, R.; Komissarov, A.A.; Chang, Y.; Liu, Y.; Matthay, M.A.
Proteolytic regulation of epithelial sodium channels by urokinase plasminogen activator: cutting edge and cleavage sites (2015), J. Biol. Chem., 290, 5241-5255.

Search for more literature for 3.4.21.73

Protein Variants

Protein Variants	Comment	Organism
additional information	three truncation mutants of the N-terminal fragment of the enzyme still activate the epithelial sodium channel	Homo sapiens
S195A	site-directed mutagenesis, the active site mutant is not able to activate the epithelial sodium channel in contrast to the wild-type enzyme	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
plasminogen activator inhibitor 1	PAI-1, complete inhibition, elevated inhibitor specifically inactivates pulmonary urokinase-type plasminogen activator and tissue.type plasminogen activator, EC 3.4.21.68	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
54000	-	x * 54000, SDS-PAGE	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
proteolytic modification	single-chain enzyme is converted to the two-chain form by incubation with immobilized plasmin, it can also be cleaved by plasmin at Lys158-Ile159 to produce potent two-chain enzyme accompanied by a conformational change in protein	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
lung	enzyme level is extremely elevated in the edematous fluid of acutely injured lungs and pleurae	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
epithelial sodium channel gamma subunit + H2O	of Xenopus laevis ocytes, activation by proteolytic cleavage at 177GR-/-KR180	Homo sapiens	?	-	?
additional information	no activity with the epithelial sodium channel alpha subunit, the enzyme shows amidolytic activity with fluorogenic substrate Pefafluor uPA	Homo sapiens	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 54000, SDS-PAGE	Homo sapiens

Synonyms

Synonyms	Comment	Organism
uPA	-	Homo sapiens
Urokinase plasminogen activator	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Homo sapiens

General Information

General Information	Comment	Organism
physiological function	activation of alphabetagamma-epithelial sodium channel specifically by the enzyme may contribute to fluid clearance under physiological conditions and in injured tissues, multifaceted mechanisms for enzyme-mediated up-regulation of the epithelial sodium channel, which form the cellular and molecular rational for the beneficial effects of urokinase in mitigating mortal pulmonary edema and pleural effusions, overview	Homo sapiens

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Release 2023.1 (January 2023)