Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | the autolysis loop in the catalytic domain of uPA is a potential inhibitory target. Three antibodies are potent inhibitors of uPA activity, two pro-uPA-specific ones by inhibiting conversion of pro-uPA to active uPA and an active uPA-specific antibody by shielding the access of plasminogen to the active site, overview. The conformation-specific antibodies mAb-112 and mAb-12E6B10 are useful to selectively stain pro-uPA or active uPA on the surface of cultured cells. Antibody mAb-112 is a non-competitive inhibitor of S-2444 cleavage by uPA with a Ki similar to the Kd for mAb-112 binding to active two-chain uPA. The epitopes of the conformation-specific antibodies are all localized to the activation and/or autolysis loop, binding kinetic analysis and models of the three-dimensional structures, overview | Homo sapiens | |
plasminogen activator inhibitor | PAI-1 | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the zymogen form pro-uPA is cleaved to the active two-chain uPA | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | pro-uPA and mature uPA | Homo sapiens | - |
U-937 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Glu-Gly-Arg-4-nitroanilide + H2O | a uPA substrate S-2444 | Homo sapiens | D-Glu-Gly-Arg + 4-nitroaniline | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | models of the three-dimensional structures of uPA and pro-uPA, and homology model of the catalytic domain of pro-uPA, overview | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
uPA | - |
Homo sapiens |
Urokinase-type plasminogen activator | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
additional information | there exists a direct link between conformational changes of the autolysis loop and the creation of a catalytically mature active site. The conformation-specific antibodies mAb-112 and mAb-12E6B10 are useful to selectively stain pro-uPA or active uPA on the surface of cultured cells | Homo sapiens |