Any feedback?
Literature summary for 3.4.21.73 extracted from
- Isolation and characterization of an inhibitory human monoclonal antibody specific to the urokinase-type plasminogen activator, uPA (2010), Protein Eng. Des. Sel., 23, 261-269.
Application
| Application | Comment | Organism |
|---|---|---|
| diagnostics | uPA is the strongest single indicator of poor prognosis in patients with metastatic breast cancer | Homo sapiens |
| pharmacology | uPA blocking antibodies may not be indicated for cancer growth inhibition strategies, but may serve as valuable tools for the implementation of pharmacodelivery strategies against a variety of different tumors | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| antibody DS2 | isolation and affinity maturation of a fully human recombinant antibody, that is specific to the human uPA and capable of inhibiting its enzymatic activity with an IC50 value in the low nanomolar range, overview. Ability of the DS2 antibody to preferentially localize at the tumor site compared with healthy organs | Homo sapiens | |
| antibody IgG(DS2) | - |
Homo sapiens | |
| antibody scFv(DS2) | - |
Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | urokinase is overexpressed in several tumors | Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Z-RRG-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | Z-RRG + 7-amino-4-methylcoumarin | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| uPA | - |
Homo sapiens |
| Urokinase | - |
Homo sapiens |
| Urokinase-type plasminogen activator | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at room temperature | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Homo sapiens |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.0000067 | - |
pH 7.4, 22°C | Homo sapiens | antibody scFv(DS2) | |
| 0.0000089 | - |
pH 7.4, 22°C | Homo sapiens | antibody IgG(DS2) |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
