Literature summary for 3.4.21.73 extracted from

Sun, Z.; Jiang, Y.; Ma, Z.; Wu, H.; Liu, B.F.; Xue, Y.; Tang, W.; Chen, Y.; Li, C.; Zhu, D.; Gurewich, V.; Liu, J.N.; Zhong, M.; Xu, Y.
Identification of a flexible loop region (297-313) of urokinase-type plasminogen activator, which helps determine its catalytic activity (1997), J. Biol. Chem., 272, 23818-23823.

Search for more literature for 3.4.21.73

Cloned(Commentary)

Cloned (Comment)	Organism
cDNA cloning	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
E301A	site-directed mutagenesis	Homo sapiens
E301D	site-directed mutagenesis	Homo sapiens
E301H	site-directed mutagenesis	Homo sapiens
K300A	site-directed mutagenesis	Homo sapiens
K300H	site-directed mutagenesis	Homo sapiens
K300W	site-directed mutagenesis	Homo sapiens
K313A	site-directed mutagenesis	Homo sapiens
S303E	site-directed mutagenesis	Homo sapiens
Y306G	site-directed mutagenesis	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.051	-	S2444	pH 7.4, 25°C, wild-type and mutant E301A, single-chain activator	Homo sapiens
0.052	-	S2444	pH 7.4, 25°C, mutant K300H, single-chain activator	Homo sapiens
0.053	-	S2444	pH 7.4, 25°C, mutant S303E, single-chain activator	Homo sapiens
0.054	-	S2444	pH 7.4, 25°C, mutant K300A and K313A, single-chain activator	Homo sapiens
0.055	-	S2444	pH 7.4, 25°C, mutant E301H, single-chain activator	Homo sapiens
0.058	-	S2444	pH 7.4, 25°C, mutant Y306G, single-chain activator	Homo sapiens
0.059	-	S2444	pH 7.4, 25°C, mutant K300W, single-chain activator	Homo sapiens
0.06	-	S2444	pH 7.4, 25°C, mutant E301D, single-chain activator	Homo sapiens
0.074	-	S2444	pH 7.4, 25°C, mutant K300W, two-chain activator	Homo sapiens
0.075	-	S2444	pH 7.4, 25°C, mutant K300H, two-chain activator	Homo sapiens
0.077	-	S2444	pH 7.4, 25°C, mutant K300A and S303E, two-chain activator	Homo sapiens
0.078	-	S2444	pH 7.4, 25°C, wild-type, two-chain activator	Homo sapiens
0.08	-	S2444	pH 7.4, 25°C, mutant K313A, two-chain activator	Homo sapiens
0.082	-	S2444	pH 7.4, 25°C, mutant E301D, two-chain activator	Homo sapiens
0.085	-	S2444	pH 7.4, 25°C, mutant Y306G, two-chain activator	Homo sapiens
0.087	-	S2444	pH 7.4, 25°C, mutant E301A, two-chain activator	Homo sapiens
0.089	-	S2444	pH 7.4, 25°C, mutant E301H, two-chain activator	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
plasminogen + H2O	Homo sapiens	principal function is fibrinolysis, u-PA also been implicated in other physiological functions such as embryogenesis, cell migration, tissue remodeling, ovulation, and wound healing	plasmin + ?	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	human	-

Purification (Commentary)

Purification (Comment)	Organism
-	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	urokinase-type plasminogen activator includes its single-chain zymogen, pro-urokinase and two-chain enzyme urokinase	Homo sapiens	?	-	?
plasminogen + H2O	-	Homo sapiens	plasmin + ?	-	?
plasminogen + H2O	principal function is fibrinolysis, u-PA also been implicated in other physiological functions such as embryogenesis, cell migration, tissue remodeling, ovulation, and wound healing	Homo sapiens	plasmin + ?	-	?
S2444 + H2O	i.e. 5-oxo-L-Pro-Gly-L-Arg-p-nitroanilide	Homo sapiens	pyroGlu-Gly-Arg + 4-nitroaniline	-	?

Synonyms

Synonyms	Comment	Organism
UK	-	Homo sapiens
Urokinase-type plasminogen activator	-	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0077	-	S2444	pH 7.4, 25°C, mutant K300A, single-chain activator	Homo sapiens
0.0085	-	S2444	pH 7.4, 25°C, mutant K300W, single-chain activator	Homo sapiens
0.027	-	S2444	pH 7.4, 25°C, mutant E301A, single-chain activator	Homo sapiens
0.029	-	S2444	pH 7.4, 25°C, mutant Y306G, single-chain activator	Homo sapiens
0.047	-	S2444	pH 7.4, 25°C, mutant E301D, single-chain activator	Homo sapiens
0.065	-	S2444	pH 7.4, 25°C, mutant K300H, single-chain activator	Homo sapiens
0.32	-	S2444	pH 7.4, 25°C, mutant S303E, single-chain activator	Homo sapiens
0.32	-	S2444	pH 7.4, 25°C, wild-type, single-chain activator	Homo sapiens
0.33	-	S2444	pH 7.4, 25°C, mutant K313A, single-chain activator	Homo sapiens
1.5	-	S2444	pH 7.4, 25°C, mutant E301H, single-chain activator	Homo sapiens
22	-	S2444	pH 7.4, 25°C, mutant Y306G, two-chain activator	Homo sapiens
27	-	S2444	pH 7.4, 25°C, mutant K300W, two-chain activator	Homo sapiens
60	-	S2444	pH 7.4, 25°C, mutant K300A, two-chain activator	Homo sapiens
83	-	S2444	pH 7.4, 25°C, mutant E301H, two-chain activator	Homo sapiens
180	-	S2444	pH 7.4, 25°C, wild-type and mutant S303E, two-chain activator	Homo sapiens
190	-	S2444	pH 7.4, 25°C, mutant K313A, two-chain activator	Homo sapiens
200	-	S2444	pH 7.4, 25°C, mutant E301A, two-chain activator	Homo sapiens
270	-	S2444	pH 7.4, 25°C, mutant E301D, two-chain activator	Homo sapiens
350	-	S2444	pH 7.4, 25°C, mutant K300H, two-chain activator	Homo sapiens

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)