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Literature summary for 3.4.21.69 extracted from

  • Deangelis, J.P.; Varfaj, F.; Wakabayashi, H.; Fay, P.J.
    The role of P4-P3 residues flanking Arg336 in facilitating activated protein C-catalyzed cleavage and inactivation of factor VIIIa (2011), Thromb. Res., 128, 470-476.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.69

Activating Compound

Activating Compound Comment Organism Structure
Phospholipid vesicles of 20% phosphatidylserine, 40% phosphatidylcholine, and 40% phosphatidylethanolamine Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information proteolysis at Arg336 occurs 25-fold faster than at Arg562. Replacing residues flanking Arg336 en bloc with the corresponding residues surrounding Arg562 markedly reduces the rate of cleavage at Arg336 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Factor Va + H2O Homo sapiens inactivation through cleavage at Arg306, Arg506, and Arg679 ?
-
 ?
Factor VIIIa + H2O Homo sapiens inactivation through cleavages at Arg336 in the A1 subunit and Arg562 in the A2 subunit ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Factor Va + H2O inactivation through cleavage at Arg306, Arg506, and Arg679 Homo sapiens ?
-
 ?
Factor VIIIa + H2O inactivation through cleavages at Arg336 in the A1 subunit and Arg562 in the A2 subunit Homo sapiens ?
-
 ?
Factor VIIIa + H2O inactivation through cleavages at Arg336 in the A1 subunit and Arg562 in the A2 subunit. Proteolysis at Arg336 occurs 25fold faster than at Arg562. Activities with substrate mutants, overview. The inactivation rates observed for point mutations at the P3 and P2 positions, Gln334Asp, Leu335Gln, and Leu335Arg, are reduced by about 2fold compared with wild-type factor VIIIa Homo sapiens ?
-
 ?
L-pGln-L-Pro-L-Arg-4-nitroanilide + H2O
-
 Homo sapiens L-pGln-L-Pro-L-Arg + 4-nitroaniline
-
 ?
additional information P4-P3' residues flanking cleavage sites in factors VIIIa and Va, overview Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
Activated protein C
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens

General Information

General Information Comment Organism
additional information residues Arg336 and Arg562 are involved in the catalytic mechanism Homo sapiens