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Literature summary for 3.4.21.69 extracted from

  • Varfaj, F.; Wakabayashi, H.; Fay, P.J.
    Residues surrounding Arg336 and Arg562 contribute to the disparate rates of proteolysis of factor VIIIa catalyzed by activated protein C (2007), J. Biol. Chem., 282, 20264-20272.
    View publication on PubMed
Search for more literature for 3.4.21.69

Activating Compound

Activating Compound Comment Organism Structure
Phospholipids
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics with wild-type and mutant factor VIIIa substrates Homo sapiens
0.0001
-
 Factor VIIIa pH 7.2, 37°C, activated protein C Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
active factor VIIIa + H2O Homo sapiens proteolytic inactivation inactive factor VIII + domain B
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 activity with wild-type and mutant factor VIIIa substrates at cleavage site Arg336 and Arg562 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
active factor VIIIa + H2O proteolytic inactivation Homo sapiens inactive factor VIII + domain B
-
 ?
active factor VIIIa + H2O proteolytic inactivation with cleavage at Arg336 within the factor VIIIa A1 subunit and Arg562 within the A2 subunit, reduced activity with three 336(P4-P3')562 fator VIII variants, possessing mutations surrounding Arg336, compared to wild-type fVIIIa, the rate of cleavage for the A1 subunit of the fully substituted P4-P3' mutant is reduced by 100fold relative to wild-type, cleavage of the A2 subunit for the 336(P4-P3')562 appear a few fold slower than that for wild-type, overview Homo sapiens inactive factor VIII + domain B
-
 ?

Synonyms

Synonyms Comment Organism
Activated protein C
-
 Homo sapiens
APC
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Homo sapiens