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Literature summary for 3.4.21.69 extracted from

  • Preston, R.J.; Ajzner, E.; Razzari, C.; Karageorgi, S.; Dua, S.; Dahlbaeck, B.; Lane, D.A.
    Multifunctional specificity of the protein C/activated protein C Gla domain (2006), J. Biol. Chem., 281, 28850-28857.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
phosphatidylcholine as phospholipid vesicles with 80% phosphatidylcholine and 20% phosphatidylserine, specific binding to the enzyme of anionic phospholipid vesicles passed over the protein C surface, defective phospholipid binding of mutant variants also resulted in severely impaired anticoagulant activity in protein C-deficient plasma Homo sapiens
phosphatidylserine as phospholipid vesicles with 80% phosphatidylcholine and 20% phosphatidylserine, specific binding to the enzyme of anionic phospholipid vesicles passed over the protein C surface, defective phospholipid binding of mutant variants also resulted in severely impaired anticoagulant activity in protein C-deficient plasma Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes in HEK-293 cells, secretion to the medium Homo sapiens

Protein Variants

Protein Variants Comment Organism
D35T/D36A site-directed mutagenesis, the mutant shows slightly increased factor Va proteolysis compared to the wild-type enzyme, the activation by protein S is reduced Homo sapiens
D36A/L38D/A39V site-directed mutagenesis, the mutant shows no activation by protein S Homo sapiens
E16D site-directed mutagenesis, the mutation causes aberrant Ca2+ binding and Gla domain misfolding Homo sapiens
N33S/V34S/D35T/D36A/L38D/A39V site-directed mutagenesis, the mutant shows slightly increased factor Va proteolysis compared to the wild-type enzyme, no activation by protein S Homo sapiens
S11G/S12N site-directed mutagenesis, the mutant shows slightly increased factor Va proteolysis compared to the wild-type enzyme Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens activated protein C has potent anticoagulant and antiinflammatory properties that are mediated in part by its interactions with its cofactor protein S and the endothelial cell protein C receptor, EPCR, the protein C/APC Gla domain is implicated in both interactions ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant enzymes from HEK-293 cell culture medium by anion exchange chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
active factor Va + H2O proteolytic inactivation Homo sapiens inactive factor V + domain B
-
?
additional information activated protein C has potent anticoagulant and antiinflammatory properties that are mediated in part by its interactions with its cofactor protein S and the endothelial cell protein C receptor, EPCR, the protein C/APC Gla domain is implicated in both interactions Homo sapiens ?
-
?
S-2238 + H2O a chromogenic substrate Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
Activated protein C
-
Homo sapiens
APC
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
protein S required, activates, the position of APC Gla domain residues is critical for protein S enhancement of APC activity and EPCR recognition, binding structure, overview Homo sapiens