Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphatidylcholine | as phospholipid vesicles with 80% phosphatidylcholine and 20% phosphatidylserine, specific binding to the enzyme of anionic phospholipid vesicles passed over the protein C surface, defective phospholipid binding of mutant variants also resulted in severely impaired anticoagulant activity in protein C-deficient plasma | Homo sapiens | |
phosphatidylserine | as phospholipid vesicles with 80% phosphatidylcholine and 20% phosphatidylserine, specific binding to the enzyme of anionic phospholipid vesicles passed over the protein C surface, defective phospholipid binding of mutant variants also resulted in severely impaired anticoagulant activity in protein C-deficient plasma | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in HEK-293 cells, secretion to the medium | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D35T/D36A | site-directed mutagenesis, the mutant shows slightly increased factor Va proteolysis compared to the wild-type enzyme, the activation by protein S is reduced | Homo sapiens |
D36A/L38D/A39V | site-directed mutagenesis, the mutant shows no activation by protein S | Homo sapiens |
E16D | site-directed mutagenesis, the mutation causes aberrant Ca2+ binding and Gla domain misfolding | Homo sapiens |
N33S/V34S/D35T/D36A/L38D/A39V | site-directed mutagenesis, the mutant shows slightly increased factor Va proteolysis compared to the wild-type enzyme, no activation by protein S | Homo sapiens |
S11G/S12N | site-directed mutagenesis, the mutant shows slightly increased factor Va proteolysis compared to the wild-type enzyme | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | activated protein C has potent anticoagulant and antiinflammatory properties that are mediated in part by its interactions with its cofactor protein S and the endothelial cell protein C receptor, EPCR, the protein C/APC Gla domain is implicated in both interactions | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from HEK-293 cell culture medium by anion exchange chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
active factor Va + H2O | proteolytic inactivation | Homo sapiens | inactive factor V + domain B | - |
? | |
additional information | activated protein C has potent anticoagulant and antiinflammatory properties that are mediated in part by its interactions with its cofactor protein S and the endothelial cell protein C receptor, EPCR, the protein C/APC Gla domain is implicated in both interactions | Homo sapiens | ? | - |
? | |
S-2238 + H2O | a chromogenic substrate | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Activated protein C | - |
Homo sapiens |
APC | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
protein S | required, activates, the position of APC Gla domain residues is critical for protein S enhancement of APC activity and EPCR recognition, binding structure, overview | Homo sapiens |