Literature summary for 3.4.21.68 extracted from

Kang, S.; Ajjappala, H.; Seo, H.; Sim, J.; Yoon, S.; Koo, B.; Kim, Y.; Lee, S.; Hahn, B.
Expression of the human tissue-plasminogen activator in hairy roots of oriental melon (Cucumis melo) (2011), Plant Mol. Biol. Rep., 29, 919-926.

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Cloned(Commentary)

Cloned (Comment)	Organism
functional expression under transcriptional control of single and multiple rooting loci promoter rolD promoters in hairy roots of Cucumis melon, the t-PA gene is integrated in the genome of hairy roots using Agrobacterium-mediated transformation of melon cotyledons. Highest levels of the recombinant t-PA accumulation in transgenic hairy roots carrying the t-PA transgene occur under the control of single and dual rolD promoters compared to triple and quadruple rolD promoters, method evaluation and optimization, overview	Homo sapiens
t-PA and synthetic t-PA genes, expression as active enzyme in Curcumis melo cv. Geumssaragi-euncheon hairy roots using Agrobacterium rhizogenes transfection, insertion of the t-PA genes in genomic DNA of transgenic hairy roots. Selection of optimum media for the mass-production of transgenic hairy root and fibrinolytic activity of cultured transgenic hairy roots using the bioreactor	Homo sapiens

Cloned (Comment)

Organism

functional expression under transcriptional control of single and multiple rooting loci promoter rolD promoters in hairy roots of Cucumis melon, the t-PA gene is integrated in the genome of hairy roots using Agrobacterium-mediated transformation of melon cotyledons. Highest levels of the recombinant t-PA accumulation in transgenic hairy roots carrying the t-PA transgene occur under the control of single and dual rolD promoters compared to triple and quadruple rolD promoters, method evaluation and optimization, overview

Homo sapiens

t-PA and synthetic t-PA genes, expression as active enzyme in Curcumis melo cv. Geumssaragi-euncheon hairy roots using Agrobacterium rhizogenes transfection, insertion of the t-PA genes in genomic DNA of transgenic hairy roots. Selection of optimum media for the mass-production of transgenic hairy root and fibrinolytic activity of cultured transgenic hairy roots using the bioreactor

Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
plasminogen activator inhibitor	PAI-1	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
59000	-	x * 59000, recombinant t-PA, SDS-PAGE	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-
Homo sapiens	-	gene t-PA	-
no activity in Cucumis melo	-	cv. Geumssaragi-euncheon, hairy roots	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	assay on fibrinolytic activity	Homo sapiens	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 59000, recombinant t-PA, SDS-PAGE	Homo sapiens

Synonyms

Synonyms	Comment	Organism
t-PA	-	Homo sapiens
Tissue plasminogen activator	-	Homo sapiens
tissue-plasminogen activator	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.8	-	assay at	Homo sapiens

General Information

General Information	Comment	Organism
physiological function	human tissue-plasminogen activator is a thrombolytic protein that plays an active role in dissolving fibrin clots by fibrinolysis and in activating plasminogen to plasmin in blood vessels	Homo sapiens
physiological function	tissue plasminogen activator is a thrombolytic protein that plays a key role in fibrinolysis by converting the plasminogen into plasmin which degrades fibrin clots in blood vessels	Homo sapiens