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Literature summary for 3.4.21.68 extracted from
- Cloning and expression of truncated form of tissue plasminogen activator in Leishmania tarentolae (2011), Biotechnol. Lett., 33, 503-508.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene K2S, expression of a His-tagged truncated enzyme form in Leishmania tarentolae using an expression cassette containing kringle 2 and serine protease domains, tissue plasminogen activator, together with a signal sequence derived from Leishmania tarentolae and two fragments of the small subunit ribosomal RNA locus. The recombinant enzyme is functional and secreted to the cell culture medium. Replacement of the human signal sequence tPA with the signal sequence derived from Leishmania increases the secretion of recombinant protein up to 30times | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 72000 | - |
x * 72000, wild-type enzyme | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| plasminogen + H2O | Homo sapiens | - |
plasmin + ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
gene K2S | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged truncated enzyme from Leishmania tarentolae by nickel affinity chromatography | Homo sapiens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
purified recombinant truncated enzyme, pH 8.3, 37°C | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| plasminogen + H2O | - |
Homo sapiens | plasmin + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 72000, wild-type enzyme | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Tissue plasminogen activator | - |
Homo sapiens |
| tPA | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.3 | - |
assay at | Homo sapiens |
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