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Literature summary for 3.4.21.62 extracted from

  • Fan, H.; Liu, Z.; Zhang, R.; Wang, N.; Dou, K.; Mijiti, G.; Diao, G.; Wang, Z.
    Functional analysis of a subtilisin-like serine protease gene from biocontrol fungus Trichoderma harzianum (2014), J. Microbiol., 52, 129-138.
    View publication on PubMed
Search for more literature for 3.4.21.62

Cloned(Commentary)

Cloned (Comment) Organism
gene ThSS45, DNA and amino acid sequence determination and analysis, phylogenetic tree, quantitative expression analysis, recombinant expression in in Escherichia coli strain BL21 Trichoderma harzianum

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Trichoderma harzianum
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44900
-
 x * 44900, about, sequence calculation, x * 69000, recombinant enzyme, SDS-PAGE Trichoderma harzianum
69000
-
 x * 44900, about, sequence calculation, x * 69000, recombinant enzyme, SDS-PAGE Trichoderma harzianum

Organism

Organism UniProt Comment Textmining
Trichoderma harzianum S5S2B8 gene ThSS45
-
Trichoderma harzianum ACCC30371 S5S2B8 gene ThSS45
-

Subunits

Subunits Comment Organism
? x * 44900, about, sequence calculation, x * 69000, recombinant enzyme, SDS-PAGE Trichoderma harzianum

Synonyms

Synonyms Comment Organism
subtilisin-like serine protease
-
 Trichoderma harzianum
ThSS45
-
 Trichoderma harzianum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 recombinant enzyme Trichoderma harzianum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 recombinant enzyme Trichoderma harzianum

pI Value

Organism Comment pI Value Maximum pI Value
Trichoderma harzianum sequence calculation
-
 5.91

Expression

Organism Comment Expression
Trichoderma harzianum transcription of gene ThSS45 is up-regulated when grown in mineral medium, under carbon starvation, and nitrogen starvation, and in the presence of 1% root powder (3.5fold), 1% stem powder, and 1% leaf powder derived from Populus davidiana × P. bolleana (Shanxin poplar) aseptic seedlings. Gene transcription of ThSS45 is also slightly up-regulated by 1% Alternaria alternata cell wall and 5% Alternaria alternata fermentation liquid up

General Information

General Information Comment Organism
evolution the enzyme is a member of the peptidases_S8/PCSK9/proteinase K-like family (Pf00082) Trichoderma harzianum
physiological function the serine protease may be involved in both mycoparasitism and antibiotic secretion Trichoderma harzianum