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Literature summary for 3.4.21.62 extracted from

  • Li, Z.; Roccatano, D.; Lorenz, M.; Martinez, R.; Schwaneberg, U.
    Insights on activity and stability of subtilisin E towards guanidinium chloride and sodium dodecylsulfate (2014), J. Biotechnol., 169, 87-94.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene aprE, recombinant expression of wild-type and mutant enzymes in Bacillus subtilis strain WB600 and secretion to the culture medium Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
N218S site-directed mutagenesis, the mutant shows increased activity and stability in the presence of GdmCl or SDS compared to the wild-type enzyme Bacillus subtilis
S62I/A153V/G166S/I205V site-directed mutagenesis, the mutant shows increased activity and stability in the presence of GdmCl or SDS compared to the wild-type enzyme Bacillus subtilis
S62I/A153V/G166S/I205V/N218S/T224A site-directed mutagenesis, the mutant shows high activity and stability in the presence of GdmCl or SDS compared to the wild-type enzyme Bacillus subtilis
S62I/A153V/G166S/T224A/T240S site-directed mutagenesis, the mutant shows high activity and stability in the presence of GdmCl or SDS compared to the wild-type enzyme Bacillus subtilis
S62I/A153V/G166S/T224A/T240S site-directed mutagenesis, the mutant shows increased activity and stability in the presence of GdmCl or SDS compared to the wild-type enzyme Bacillus subtilis
T224A site-directed mutagenesis, the mutant shows increased activity and stability in the presence of GdmCl or SDS compared to the wild-type enzyme Bacillus subtilis

General Stability

General Stability Organism
stability of wild-type an dmutant enzymes in the presence of GdmCl or SDS, overview Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
guanidinium hydrochloride
-
Bacillus subtilis
SDS
-
Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis P04189 gene aprE
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant enzymes from Bacillus subtilis strain WB600 culture supernatant by anion and cation exchange chromatography and ultrafilatration Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme shows activity towards complex substrates, e.g. skimmed milk Bacillus subtilis ?
-
?
succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-methyl-coumaryl-7-amide + H2O
-
Bacillus subtilis succinyl-L-Ala-L-Ala-L-Pro-L-Phe + 7-amino-4-methylcoumarin
-
?

Synonyms

Synonyms Comment Organism
Subtilisin E
-
Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
assay at Bacillus subtilis

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
700
-
pH 7.5, 37°C, recombinant wild-type enzyme Bacillus subtilis guanidinium hydrochloride
960
-
pH 7.5, 37°C, recombinant mutant N218S Bacillus subtilis guanidinium hydrochloride
1260
-
pH 7.5, 37°C, recombinant mutant T224A Bacillus subtilis guanidinium hydrochloride
2730
-
pH 7.5, 37°C, recombinant mutant S62I/A153V/G166S/I205V Bacillus subtilis guanidinium hydrochloride
3070
-
pH 7.5, 37°C, recombinant mutant S62I/A153V/G166S/T224A/T240S Bacillus subtilis guanidinium hydrochloride
4650
-
pH 7.5, 37°C, recombinant mutant S62I/A153V/G166S/I205V/N218S/T224A Bacillus subtilis guanidinium hydrochloride