Cloned (Comment) | Organism |
---|---|
gene aprE, recombinant expression of wild-type and mutant enzymes in Bacillus subtilis strain WB600 and secretion to the culture medium | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
N218S | site-directed mutagenesis, the mutant shows increased activity and stability in the presence of GdmCl or SDS compared to the wild-type enzyme | Bacillus subtilis |
S62I/A153V/G166S/I205V | site-directed mutagenesis, the mutant shows increased activity and stability in the presence of GdmCl or SDS compared to the wild-type enzyme | Bacillus subtilis |
S62I/A153V/G166S/I205V/N218S/T224A | site-directed mutagenesis, the mutant shows high activity and stability in the presence of GdmCl or SDS compared to the wild-type enzyme | Bacillus subtilis |
S62I/A153V/G166S/T224A/T240S | site-directed mutagenesis, the mutant shows high activity and stability in the presence of GdmCl or SDS compared to the wild-type enzyme | Bacillus subtilis |
S62I/A153V/G166S/T224A/T240S | site-directed mutagenesis, the mutant shows increased activity and stability in the presence of GdmCl or SDS compared to the wild-type enzyme | Bacillus subtilis |
T224A | site-directed mutagenesis, the mutant shows increased activity and stability in the presence of GdmCl or SDS compared to the wild-type enzyme | Bacillus subtilis |
General Stability | Organism |
---|---|
stability of wild-type an dmutant enzymes in the presence of GdmCl or SDS, overview | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
guanidinium hydrochloride | - |
Bacillus subtilis | |
SDS | - |
Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | P04189 | gene aprE | - |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from Bacillus subtilis strain WB600 culture supernatant by anion and cation exchange chromatography and ultrafilatration | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme shows activity towards complex substrates, e.g. skimmed milk | Bacillus subtilis | ? | - |
? | |
succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-methyl-coumaryl-7-amide + H2O | - |
Bacillus subtilis | succinyl-L-Ala-L-Ala-L-Pro-L-Phe + 7-amino-4-methylcoumarin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Subtilisin E | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Bacillus subtilis |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
700 | - |
pH 7.5, 37°C, recombinant wild-type enzyme | Bacillus subtilis | guanidinium hydrochloride | |
960 | - |
pH 7.5, 37°C, recombinant mutant N218S | Bacillus subtilis | guanidinium hydrochloride | |
1260 | - |
pH 7.5, 37°C, recombinant mutant T224A | Bacillus subtilis | guanidinium hydrochloride | |
2730 | - |
pH 7.5, 37°C, recombinant mutant S62I/A153V/G166S/I205V | Bacillus subtilis | guanidinium hydrochloride | |
3070 | - |
pH 7.5, 37°C, recombinant mutant S62I/A153V/G166S/T224A/T240S | Bacillus subtilis | guanidinium hydrochloride | |
4650 | - |
pH 7.5, 37°C, recombinant mutant S62I/A153V/G166S/I205V/N218S/T224A | Bacillus subtilis | guanidinium hydrochloride |