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Literature summary for 3.4.21.62 extracted from

  • Kim, J.; Kim, J.H.; Choi, K.H.; Kim, J.H.; Song, Y.S.; Cha, J.
    Enhancement of the catalytic activity of a 27 kDa subtilisin-like enzyme from Bacillus amyloliquefaciens CH51 by in vitro mutagenesis (2011), J. Agric. Food Chem., 59, 8675-8682.
    View publication on PubMed
Search for more literature for 3.4.21.62

Cloned(Commentary)

Cloned (Comment) Organism
expressed in the protease deficient host Bacillus subtilis ISW1214 Bacillus amyloliquefaciens

Protein Variants

Protein Variants Comment Organism
G169A mutant shows 1.4fold activity compared to wild-type Bacillus amyloliquefaciens
S101K/G169A mutant shows 3.3fold activity compared to wild-type Bacillus amyloliquefaciens
S101L/G169A mutant shows 2.9fold activity compared to wild-type Bacillus amyloliquefaciens
S101R/G169A mutant shows 2.2fold activity compared to wild-type Bacillus amyloliquefaciens
S101W/G169A mutant shows 3.9fold activity compared to wild-type Bacillus amyloliquefaciens
S101W/G169A/V192A mutant shows 1.4fold activity compared to wild-type, kcat doubled compared to wild-type, Km decreased compared to wild-type Bacillus amyloliquefaciens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
 N-Succ-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.4, 37°C, mutant S101W/G169A/V192A Bacillus amyloliquefaciens
0.35
-
 N-Succ-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.4, 37°C, wild-type Bacillus amyloliquefaciens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
-
 Bacillus amyloliquefaciens

Organism

Organism UniProt Comment Textmining
Bacillus amyloliquefaciens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using a HiTrap Q HP column Bacillus amyloliquefaciens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
casein + H2O caseinolytic activity is determined using the casein plate method Bacillus amyloliquefaciens ?
-
 ?
Human fibrinogen + H2O fibrinolytic activity is determined using the fibrin plate method Bacillus amyloliquefaciens ?
-
 ?
N-Succ-Ala-Ala-Pro-Phe-4-nitroanilide + H2O
-
 Bacillus amyloliquefaciens N-Succ-Ala-Ala-Pro-Phe + 4-nitroaniline
-
 ?

Synonyms

Synonyms Comment Organism
AprE51
-
 Bacillus amyloliquefaciens
subtilisin A
-
 Bacillus amyloliquefaciens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Bacillus amyloliquefaciens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
45 50 optimal reaction Bacillus amyloliquefaciens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 remarkable loss of activity at temperature higher than 50°C Bacillus amyloliquefaciens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
231.3
-
 N-Succ-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.4, 37°C, wild-type Bacillus amyloliquefaciens
586.2
-
 N-Succ-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.4, 37°C, mutant S101W/G169A/V192A Bacillus amyloliquefaciens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Bacillus amyloliquefaciens

pH Range

pH Minimum pH Maximum Comment Organism
7 11 optimal reaction Bacillus amyloliquefaciens

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 12 enzyme loses its activity at pH lower 4, retains 60% of its activity at pH higher 12 Bacillus amyloliquefaciens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
660
-
 N-Succ-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.4, 37°C, wild-type Bacillus amyloliquefaciens
3910
-
 N-Succ-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.4, 37°C, mutant S101W/G169A/V192A Bacillus amyloliquefaciens