Literature summary for 3.4.21.62 extracted from

Castillo, B.; Sola, R.J.; Ferrer, A.; Barletta, G.; Griebenow, K.
Effect of PEG modification on subtilisin Carlsberg activity, enantioselectivity, and structural dynamics in 1,4-dioxane (2008), Biotechnol. Bioeng., 99, 9-17.

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Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	improvement in the enzymatic activity for the poly(ethylene)-glycol-modified enzyme in the organic solvent that depends on the level of poly(ethylene)-glycolylation. PEGSBc conjugates with 1.1, 1.9, and 3.2 average mol of poly(ethylene)-glycol per mol of protein reveal a 30fold, 57fold, and 99fold increase in enzymatic activity as a result of poly(ethylene)-glycolylation	Bacillus licheniformis

Application

Application	Comment	Organism
additional information	ability of poly(ethylene)-glycol-modification to systematically modulate the structural dynamics of subtilisin Carlsberg in 1,4-dioxane. At increasing levels of modification the enzyme becomes more flexible, thus catalytically more active. In contrast, increasing the enzyme flexibility through poly(ethylene)-glycol-modification reduces the enzyme enantioselectivity	Bacillus licheniformis

Inhibitors

Inhibitors	Comment	Organism	Structure
1,4-dioxane	104fold decrease in the enzymes catalytic activity for the hydrolysis reaction of vinyl butyrate with D2O and a 50% decrease in enzyme structural dynamics. Attaching increasing amounts of PEG to the enzyme reverses some of the activity loss. Poly(ethylene)-glycolylation increases protein structural dynamics in 1,4-dioxane	Bacillus licheniformis

Organism

Organism	UniProt	Comment	Textmining
Bacillus licheniformis	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
vinyl butyrate + H2O	-	Bacillus licheniformis	?	-	?

Synonyms

Synonyms	Comment	Organism
SBc	-	Bacillus licheniformis
subtilisin Carlsberg	-	Bacillus licheniformis

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Release 2023.1 (January 2023)