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Literature summary for 3.4.21.62 extracted from

  • Fisher, K.E.; Ruan, B.; Alexander, P.A.; Wang, L.; Bryan, P.N.
    Mechanism of the kinetically-controlled folding reaction of subtilisin (2007), Biochemistry, 46, 640-651.
    View publication on PubMed
Search for more literature for 3.4.21.62

Activating Compound

Activating Compound Comment Organism Structure
additional information prodomain mutant proR9 accelerates subtilisin folding. Subtilisin folding with the wild-type prodomain is slow. The wild-type prodomain binds 100times slower than proR9 Bacillus amyloliquefaciens

Application

Application Comment Organism
additional information slow folding of subtilisin results from the formation of an unstable and topologically challenged intermediate and the proline-limited isomerization of the intermediate to the native state Bacillus amyloliquefaciens

Cloned(Commentary)

Cloned (Comment) Organism
inactive mutants of subtilisin BPN’ expressed in Bacillus subtilis. Expression of prodomain mutant proR9 in Escherichia coli strain BL21(DE3) Bacillus amyloliquefaciens

Protein Variants

Protein Variants Comment Organism
D32N does not fold at all in the presence of proR9 Bacillus amyloliquefaciens
N155L folding kinetics very similar to that of S221A Bacillus amyloliquefaciens
P168G weaker binding of proR9 Bacillus amyloliquefaciens
S221A a delta75-83 mutant. Shows slow folding reaction. Is unstable at low metal concentration, unfolding at a rate of 0.6 h-1. If denatured, it is refolded with an excess of proR9 Bacillus amyloliquefaciens
S221C folding kinetics very similar to that of S221A Bacillus amyloliquefaciens
T22C/S87C/S221C if denatured, it is refolded with an excess of proR9. About 75% is rapidly bound to proR9. 25% undergoes a slow step prior to binding Bacillus amyloliquefaciens

Metals/Ions

Metals/Ions Comment Organism Structure
additional information metal ion sites are not formed until late in folding and do not influence the kinetics of folding to the intermediate complex Bacillus amyloliquefaciens

Organism

Organism UniProt Comment Textmining
Bacillus amyloliquefaciens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus amyloliquefaciens

Storage Stability

Storage Stability Organism
-20°C Bacillus amyloliquefaciens

Synonyms

Synonyms Comment Organism
subtilisin BPN’
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 Bacillus amyloliquefaciens