Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibitory: Tween-20, Tween-40, Tween-60, Tween-80 at 1%, sodium dodecylsulfate at 0.2%, for 1 h at 30°C. Not inhibitory: iodoacetate, ethylacetimide, phenylglyoxal, N-ethylmaleimide, N-bromosuccinimide | Alkalihalobacillus clausii | |
phenylmethylsulfonyl fluoride | 1 mM, 82% inhibition | Alkalihalobacillus clausii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000655 | - |
N-succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide | 37°C, pH 10.5 | Alkalihalobacillus clausii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 5 mM, 40% increase in activity | Alkalihalobacillus clausii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26500 | - |
x * 26500, SDS-PAGE | Alkalihalobacillus clausii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alkalihalobacillus clausii | - |
highest activity in late stationary growth phase in protein-rich medium at pH 10.5 | - |
Alkalihalobacillus clausii GMBAE 42 | - |
highest activity in late stationary growth phase in protein-rich medium at pH 10.5 | - |
Purification (Comment) | Organism |
---|---|
from culture filtrate | Alkalihalobacillus clausii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6852 | - |
37°C, pH 10.5 | Alkalihalobacillus clausii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Hammarsten casein + H2O | activation energy for hydrolysis 10.59 kcal per mol | Alkalihalobacillus clausii | ? | - |
? | |
Hammarsten casein + H2O | activation energy for hydrolysis 10.59 kcal per mol | Alkalihalobacillus clausii GMBAE 42 | ? | - |
? | |
N-succinyl-L-Ala-L-Ala-L-L-Ala-4-nitroanilide + H2O | 3.8% of the activity with N-succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide | Alkalihalobacillus clausii | ? | - |
? | |
N-succinyl-L-Ala-L-Ala-L-L-Ala-4-nitroanilide + H2O | 3.8% of the activity with N-succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide | Alkalihalobacillus clausii GMBAE 42 | ? | - |
? | |
N-succinyl-L-Ala-L-Ala-L-Pro-L-Leu-4-nitroanilide + H2O | 19% of the activity with N-succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide | Alkalihalobacillus clausii | ? | - |
? | |
N-succinyl-L-Ala-L-Ala-L-Pro-L-Leu-4-nitroanilide + H2O | 19% of the activity with N-succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide | Alkalihalobacillus clausii GMBAE 42 | ? | - |
? | |
N-succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide + H2O | - |
Alkalihalobacillus clausii | ? | - |
? | |
N-succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide + H2O | - |
Alkalihalobacillus clausii GMBAE 42 | ? | - |
? | |
N-succinyl-L-Ala-L-Ala-L-Val-4-nitroanilide + H2O | 2.2% of the activity with N-succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide | Alkalihalobacillus clausii | ? | - |
? | |
N-succinyl-L-Ala-L-Ala-L-Val-4-nitroanilide + H2O | 2.2% of the activity with N-succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide | Alkalihalobacillus clausii GMBAE 42 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 26500, SDS-PAGE | Alkalihalobacillus clausii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
in absence of Ca2+ | Alkalihalobacillus clausii |
70 | - |
in presence of 5 mM Ca2+ | Alkalihalobacillus clausii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 40 | pH 10.5, stable for 2 h | Alkalihalobacillus clausii |
50 | - |
pH 10.5, 2 h, 85% residual activity | Alkalihalobacillus clausii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
35.5 | - |
Hammarsten casein | 37°C, pH 10.5 | Alkalihalobacillus clausii | |
70.2 | - |
N-succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide | 37°C, pH 10.5 | Alkalihalobacillus clausii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
11.3 | - |
- |
Alkalihalobacillus clausii |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
1 | 3 | 24 h, 76% loss of activity | Alkalihalobacillus clausii |
9 | 12.2 | 30°C, stable for 24 h | Alkalihalobacillus clausii |
12.7 | - |
24 h, 38% loss of activity | Alkalihalobacillus clausii |