Cloned (Comment) | Organism |
---|---|
expression of full-length enzyme and mature form, expression of mature form does not give enzymatic activity | Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
x * 29000, mature enzyme | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | Q6IT79 | proenzyme | - |
Bacillus sp. (in: Bacteria) DJ-4 | Q6IT79 | proenzyme | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Fibrinogen + H2O | rapid hydrolysis of Aalpha-, Bbeta- and gamma-chains. At very low concentration, no cleavage of gamma-chain | Bacillus sp. (in: Bacteria) | ? | - |
? | |
Fibrinogen + H2O | rapid hydrolysis of Aalpha-, Bbeta- and gamma-chains. At very low concentration, no cleavage of gamma-chain | Bacillus sp. (in: Bacteria) DJ-4 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 29000, mature enzyme | Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
recombinant enzyme, stable for 40 min, native enzyme, 30% loss of activity after 40 min | Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | broad | Bacillus sp. (in: Bacteria) |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | - |
recombinant enzyme, about 85% residual activity after 48 h at 4°C, native enzyme, 60% residual activity | Bacillus sp. (in: Bacteria) |