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Literature summary for 3.4.21.62 extracted from
- Purification and characterization of serine proteinase 2 from Bacillus intermedius 3-19 (2004), Biochemistry (Moscow), 69, 420-426.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diisopropyl fluorophosphate | - |
Bacillus intermedius |  |
| duck ovomucoid | - |
Bacillus intermedius | |
| Hg2+ | - |
Bacillus intermedius | |
| p-chloromercuribenzoate | partial inhibition | Bacillus intermedius | |
| phenylmethylsulfonyl fluoride | - |
Bacillus intermedius | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0054 | - |
benzyloxycarbonyl-L-Ala-L-Ala-L-Leu-4-nitroanilide | - |
Bacillus intermedius | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | increase on activity by 20% | Bacillus intermedius | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
x * 28000, SDS-PAGE | Bacillus intermedius |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus intermedius | - |
strain 3-19 | - |
| Bacillus intermedius Mrz 19 | - |
strain 3-19 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus intermedius |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture fluid | - |
Bacillus intermedius | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzyloxycarbonyl-L-Ala-L-Ala-L-Leu-4-nitroanilide + H2O | - |
Bacillus intermedius | benzyloxycarbonyl-L-Ala-L-Ala-L-Leu + 4-nitroaniline | - |
? | |
| benzyloxycarbonyl-L-Ala-L-Ala-L-Leu-4-nitroanilide + H2O | - |
Bacillus intermedius Mrz 19 | benzyloxycarbonyl-L-Ala-L-Ala-L-Leu + 4-nitroaniline | - |
? | |
| casein + H2O | - |
Bacillus intermedius | ? | - |
? | |
| casein + H2O | - |
Bacillus intermedius Mrz 19 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 28000, SDS-PAGE | Bacillus intermedius |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
in presence of Ca2+ | Bacillus intermedius |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | 45 | presence of Ca2+ | Bacillus intermedius |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 16550 | - |
benzyloxycarbonyl-L-Ala-L-Ala-L-Leu-4-nitroanilide | - |
Bacillus intermedius | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
substrate casein | Bacillus intermedius |
| 9 | - |
substrate benzyloxycarbonyl-L-Ala-L-Ala-L-Leu-4-nitroanilide | Bacillus intermedius |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.3 | 9.5 | - |
Bacillus intermedius |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Bacillus intermedius | isoelectric focusing | - |
9.2 |
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