Cloned (Comment) | Organism |
---|---|
AkP gene cloned and expressed in Escherichia coli PB5517 | Bacillus sp. (in: Bacteria) |
Crystallization (Comment) | Organism |
---|---|
crystal structure determined by molecular replacement, crystals grown in hanging drops, monoclinic space group P2(1), with cell dimensions a : 44.1 A, b : 51.7 A, c : 52.8 A, coordinates and structure factor amplitudes in the ProteinDataBank codes 1DBI and R1DBISF | Bacillus sp. (in: Bacteria) |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | bound calcium ions play a key role in protecting against autolysis and thermal denaturation | Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27800 | - |
calculated from the sequence | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | Q45670 | strain Ak.1 | - |
Bacillus sp. (in: Bacteria) Ak1 | Q45670 | strain Ak.1 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Bacillus sp. (in: Bacteria) |
Synonyms | Comment | Organism |
---|---|---|
Ak.1 protease | - |
Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 105 | highly thermostable, heat-treating for 2 h at 70°C activates the protease, half-life at 85°C in presence of DTT is 34 min, in absence of DTT 317 min, at 105°C complete loss of activity is virtually instantaneous in absence of sorbitol, in presence of 90% sorbitol the half-life is 104 min | Bacillus sp. (in: Bacteria) |