Protein Variants | Comment | Organism |
---|---|---|
S166C | site-directed mutagenesis combined with chemical modification | Lederbergia lentus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.68 | - |
succinyl-AAPF-p-nitroanilide | pH 8.6, 25°C, mutant S166C-a | Lederbergia lentus | |
0.7 | - |
succinyl-AAPF-p-nitroanilide | pH 8.6, 25°C, mutant S166C-f | Lederbergia lentus | |
0.73 | - |
succinyl-AAPF-p-nitroanilide | pH 8.6, 25°C wild-type | Lederbergia lentus | |
0.74 | - |
succinyl-AAPF-p-nitroanilide | pH 8.6, 25°C, mutant S166C-g | Lederbergia lentus | |
0.8 | - |
succinyl-AAPA-p-nitroanilide | pH 8.6, 25°C mutant S166C-S-c | Lederbergia lentus | |
1.09 | - |
succinyl-AAPF-p-nitroanilide | pH 8.6, 25°C mutant S166C-S-e | Lederbergia lentus | |
1.17 | - |
succinyl-AAPF-p-nitroanilide | pH 8.6, 25°C mutant S166C-S-c | Lederbergia lentus | |
1.34 | - |
succinyl-AAPF-p-nitroanilide | pH 8.6, 25°C mutant S166C-S-b | Lederbergia lentus | |
1.5 | 2 | succinyl-AAPF-p-nitroanilide | pH 8.6, 25°C mutant S166C-S-h | Lederbergia lentus | |
1.6 | - |
succinyl-AAPF-p-nitroanilide | pH 8.6, 25°C mutant S166C-S-d | Lederbergia lentus | |
1.74 | - |
succinyl-AAPA-p-nitroanilide | pH 8.6, 25°C mutant S166C-S-g | Lederbergia lentus | |
1.9 | - |
succinyl-AAPE-p-nitroanilide | pH 8.6, 25°C mutant S166C-S-a | Lederbergia lentus | |
1.9 | - |
succinyl-AAPA-p-nitroanilide | pH 8.6, 25°C mutant S166C-S-e and S166S-f | Lederbergia lentus | |
2 | - |
succinyl-AAPA-p-nitroanilide | pH 8.6, 25°C wild-type | Lederbergia lentus | |
2.26 | - |
succinyl-AAPF-p-nitroanilide | pH 8.6, 25°C mutant S166C-S-i | Lederbergia lentus | |
2.46 | - |
succinyl-AAPF-p-nitroanilide | pH 8.6, 25°C mutant S166C-S-j | Lederbergia lentus | |
3.4 | - |
succinyl-AAPR-p-nitroanilide | pH 8.6, 25°C mutant S166C-S-b | Lederbergia lentus | |
4.4 | - |
succinyl-AAPE-p-nitroanilide | pH 8.6, 25°C wild-type | Lederbergia lentus | |
5.2 | - |
succinyl-AAPR-p-nitroanilide | pH 8.6, 25°C mutant S166C-S-j | Lederbergia lentus | |
5.3 | - |
succinyl-AAPR-p-nitroanilide | pH 8.6, 25°C mutant S166C-S-i | Lederbergia lentus | |
5.5 | - |
succinyl-AAPR-p-nitroanilide | pH 8.6, 25°C mutant S166C-S-d | Lederbergia lentus | |
7.2 | - |
succinyl-AAPR-p-nitroanilide | pH 8.6, 25°C wild-type | Lederbergia lentus | |
8.2 | - |
succinyl-AAPR-p-nitroanilide | pH 8.6, 25°C mutant S166C-S-h | Lederbergia lentus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lederbergia lentus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
succinyl-AAPA-p-nitroanilide + H2O | - |
Lederbergia lentus | ? | - |
? | |
succinyl-AAPE-p-nitroanilide + H2O | - |
Lederbergia lentus | ? | - |
? | |
succinyl-AAPF-4-nitroanilide + H2O | - |
Lederbergia lentus | succinyl-AAPF + 4-nitroaniline | - |
? | |
succinyl-AAPR-p-nitroanilide + H2O | - |
Lederbergia lentus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
EC 3.4.21.14 | formerly included in | Lederbergia lentus |
SBL | - |
Lederbergia lentus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.052 | 2.1 | succinyl-AAPA-p-nitroanilide | pH 8.6, 25°C mutant S166C-S-g | Lederbergia lentus | |
0.16 | - |
succinyl-AAPR-p-nitroanilide | pH 8.6, 25°C wild-type | Lederbergia lentus | |
0.17 | - |
succinyl-AAPR-p-nitroanilide | pH 8.6, 25°C mutant S166C-S-b | Lederbergia lentus | |
0.35 | - |
succinyl-AAPR-p-nitroanilide | pH 8.6, 25°C mutant S166C-S-h | Lederbergia lentus | |
0.43 | - |
succinyl-AAPR-p-nitroanilide | pH 8.6, 25°C mutant S166C-S-i | Lederbergia lentus | |
0.68 | - |
succinyl-AAPR-p-nitroanilide | pH 8.6, 25°C mutant S166C-S-d | Lederbergia lentus | |
1.06 | - |
succinyl-AAPR-p-nitroanilide | pH 8.6, 25°C mutant S166C-S-j | Lederbergia lentus | |
1.75 | - |
succinyl-AAPE-p-nitroanilide | pH 8.6, 25°C wild-type | Lederbergia lentus | |
4.8 | - |
succinyl-AAPF-p-nitroanilide | pH 8.6, 25°C mutant S166C-S-f | Lederbergia lentus | |
6.08 | - |
succinyl-AAPR-p-nitroanilide | pH 8.6, 25°C mutant S166C-S-d | Lederbergia lentus | |
6.08 | - |
succinyl-AAPR-p-nitroanilide | pH 8.6, 25°C mutant S166C-S-j | Lederbergia lentus | |
6.8 | - |
succinyl-AAPA-p-nitroanilide | pH 8.6, 25°C mutant S166C-S-c and S166C-S-e | Lederbergia lentus | |
9.65 | - |
succinyl-AAPA-p-nitroanilide | pH 8.6, 25°C mutant S166C-S-g | Lederbergia lentus | |
14.5 | - |
succinyl-AAPE-p-nitroanilide | pH 8.6, 25°C mutant S166C-S-a | Lederbergia lentus | |
16.9 | - |
succinyl-AAPF-p-nitroanilide | pH 8.6, 25°C mutant S166C-S-c | Lederbergia lentus | |
17.7 | - |
succinyl-AAPA-p-nitroanilide | pH 8.6, 25°C wild-type | Lederbergia lentus | |
23.1 | - |
succinyl-AAPF-p-nitroanilide | pH 8.6, 25°C mutant S166C-S-c | Lederbergia lentus | |
25 | - |
succinyl-AAPF-p-nitroanilide | pH 8.6, 25°C mutant S166C-S-b | Lederbergia lentus | |
28.2 | - |
succinyl-AAPA-p-nitroanilide | pH 8.6, 25°C mutant S166C-S-f | Lederbergia lentus | |
29 | - |
succinyl-AAPF-p-nitroanilide | pH 8.6, 25°C mutant S166C-S-g | Lederbergia lentus | |
47 | - |
succinyl-AAPF-p-nitroanilide | pH 8.6, 25°C mutant S166C-S-d | Lederbergia lentus | |
48 | - |
succinyl-AAPF-p-nitroanilide | pH 8.6, 25°C, mutant S166C-h | Lederbergia lentus | |
50 | - |
succinyl-AAPF-p-nitroanilide | pH 8.6, 25°C mutant S166C-S-a | Lederbergia lentus | |
67 | - |
succinyl-AAPF-p-nitroanilide | pH 8.6, 25°C mutant S166C-S-i | Lederbergia lentus | |
76 | - |
succinyl-AAPF-p-nitroanilide | pH 8.6, 25°C mutant S166C-S-j | Lederbergia lentus | |
82 | - |
succinyl-AAPF-p-nitroanilide | pH 8.6, 25°C mutant S166C-S-e | Lederbergia lentus | |
153 | - |
succinyl-AAPF-p-nitroanilide | pH 8.6, 25°C wild-type | Lederbergia lentus |