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Literature summary for 3.4.21.62 extracted from

  • DeSantis, G.; Berglund, P.; Stabile, M.R.; Gold, M.; Jones, J.B.
    Site-directed mutagenesis combined with chemical modification as a strategy for altering the specificity of the S1 an S1'pockets of subtilisin Bacillus lentus (1998), Biochemistry, 37, 5968-5973.
    View publication on PubMed
Search for more literature for 3.4.21.62

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Lederbergia lentus

Protein Variants

Protein Variants Comment Organism
M222C site-directed mutagenesis combined with chemical modification Lederbergia lentus
S156C site-directed mutagenesis combined with chemical modification Lederbergia lentus
S166C site-directed mutagenesis combined with chemical modification Lederbergia lentus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.39
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S166C-S-a Lederbergia lentus
0.51
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S166C Lederbergia lentus
0.54
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S156C-S-d Lederbergia lentus
0.55
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C, wild-type and mutant M222C-S-e Lederbergia lentus
0.6
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S156C-S-c and S166C-S-f Lederbergia lentus
0.61
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant M222C-S-f Lederbergia lentus
0.63
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant M222C-S-b Lederbergia lentus
0.65
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S156C Lederbergia lentus
0.67
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant M222C-S-d Lederbergia lentus
0.68
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S156C-S-b and M222-S-a Lederbergia lentus
0.69
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant M222C-S-c Lederbergia lentus
0.7
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S166C-S-e Lederbergia lentus
0.72
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S166C-S-c Lederbergia lentus
0.74
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S166C-S-d Lederbergia lentus
0.77
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant M222C Lederbergia lentus
0.78
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S156C-S-e Lederbergia lentus
0.79
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S156C-S-a Lederbergia lentus
0.86
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S156C-S-f Lederbergia lentus
0.92
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S166C-S-b Lederbergia lentus

Organism

Organism UniProt Comment Textmining
Lederbergia lentus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and mutant enzymes Lederbergia lentus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
succinyl-AAPF-p-nitroanilide + H2O
-
 Lederbergia lentus succinyl-AAPF + p-nitroaniline
-
 ?

Synonyms

Synonyms Comment Organism
EC 3.4.21.14 formerly included in Lederbergia lentus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.017
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant M222C-S-a Lederbergia lentus
0.49
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant M222C-S-c Lederbergia lentus
0.833
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S166C-S-f Lederbergia lentus
1.04
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant M222C-S-f Lederbergia lentus
1.64
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant M222C-S-e Lederbergia lentus
2.94
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant M222C-S-e Lederbergia lentus
3.7
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S156C-S-e Lederbergia lentus
3.8
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S166C-S-e Lederbergia lentus
6.08
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant M222C-S-f Lederbergia lentus
6.9
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S166C-S-d and M222C-S-d Lederbergia lentus
7.2
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant M222C-S-b Lederbergia lentus
11.8
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S166C-S-b Lederbergia lentus
14.2
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S166C Lederbergia lentus
16.3
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S166C-S-f Lederbergia lentus
17.3
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant M222C Lederbergia lentus
17.4
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S166C-S-a Lederbergia lentus
18
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S156C-S-c Lederbergia lentus
21.8
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S156C-S-d Lederbergia lentus
23.4
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S156C-S-b Lederbergia lentus
24.2
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S156C-S-c Lederbergia lentus
30
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S156C-S-a Lederbergia lentus
31
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S166C-S-c Lederbergia lentus
31.6
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S156C-S-e Lederbergia lentus
33.3
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant M222C-S-a Lederbergia lentus
39
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S156C-S-f Lederbergia lentus
43
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S156C Lederbergia lentus
48
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C, wild-type Lederbergia lentus