Cloned (Comment) | Organism |
---|---|
- |
Bacillus sp. (in: Bacteria) |
- |
Bacillus amyloliquefaciens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
succinyl-Ala-Ala-Pro-Phe 4-nitroanilide | - |
Bacillus sp. (in: Bacteria) | |
0.13 | - |
succinyl-Ala-Ala-Pro-Phe 4-nitroanilide | mutant subtilisin BPN' Asp to Ser99 | Bacillus amyloliquefaciens | |
0.15 | - |
MeO-succinyl-Ala-Ala-Phe 4-nitroanilide | - |
Bacillus sp. (in: Bacteria) | |
0.15 | - |
MeO-succinyl-Ala-Ala-Phe 4-nitroanilide | succinyl-Ala-Ala-Pro-Phe 4-nitroanilide, subtilisin BPN' | Bacillus amyloliquefaciens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus amyloliquefaciens | - |
wild-type and mutant (Asp to Ser99), the wild-type subtilisin BPN' is expressed and secreted by Bacillus subtilis DB104 | - |
Bacillus sp. (in: Bacteria) | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
MeO-succinyl-Ala-Ala-Phe 4-nitroanilide + H2O | - |
Bacillus sp. (in: Bacteria) | ? | - |
? | |
MeO-succinyl-Ala-Ala-Phe 4-nitroanilide + H2O | - |
Bacillus amyloliquefaciens | ? | - |
? | |
succinyl-Ala-Ala-Pro-Phe 4-nitroanilide + H2O | - |
Bacillus sp. (in: Bacteria) | succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline | - |
? | |
succinyl-Ala-Ala-Pro-Phe 4-nitroanilide + H2O | - |
Bacillus amyloliquefaciens | succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
45 | - |
succinyl-Ala-Ala-Pro-Phe 4-nitroanilide | mutant subtilisin BPN' Asp99Ser | Bacillus amyloliquefaciens | |
57 | - |
MeO-succinyl-Ala-Ala-Phe 4-nitroanilide | subtilisin Carlsberg | Bacillus sp. (in: Bacteria) | |
57 | - |
MeO-succinyl-Ala-Ala-Phe 4-nitroanilide | subtilisin Carlsberg | Bacillus amyloliquefaciens | |
57 | - |
succinyl-Ala-Ala-Pro-Phe 4-nitroanilide | subtilisin BPN' | Bacillus sp. (in: Bacteria) | |
57 | - |
succinyl-Ala-Ala-Pro-Phe 4-nitroanilide | subtilisin BPN' | Bacillus amyloliquefaciens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Bacillus sp. (in: Bacteria) |
additional information | - |
mutant subtilisin Asp to Ser99, change of just one surface charge which is 14-15 A from the active site of subtilisin has a significant effect on the pH-dependence | Bacillus amyloliquefaciens |