Crystallization (Comment) | Organism |
---|---|
purified alpha-thrombin complexed with a modified 15-mer thrombin-binding aptamer, sitting drop vapour diffusion method, 8 mg/ml protein in 50 mM potassium phosphate, pH 7.1, and 0.1 M KCl, is mixed in a 1:1 ratio of 0.001 ml each with crystallization solution, containing 20% v/v PEG 20 000, 0.2 M ammonium sulfate, 3% v/v n-propanol, 100 mM sodium acetate, pH 5.8, 20°C, a few days, X-ray diffraction structure determination and analysis at 2.15 A resolution | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
thrombin-binding aptamer | a consensus DNA 15-mer that binds specifically to human alpha-thrombin at nanomolar concentrations and inhibits its procoagulant functions. A a modified thrombin-binding aptamer, containing a 5'-5' inversion-of-polarity site, is more stable and to possesses a higher thrombin affinity than its unmodified counterpart | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Synonyms | Comment | Organism |
---|---|---|
alpha-thrombin | - |
Homo sapiens |