Crystallization (Comment) | Organism |
---|---|
mutant enzyme N143P is crystallized by the hanging drop vapor diffusion method, using 0.2 M NaNO3 and 20% (w/v) PEG3350 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
N143P t | the mutant features no Na+-dependent enhancement of kcat yet binds Na+ with an affinity comparable to that of wild type | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00033 | - |
D-Phe-Pro-Arg-4-nitroanilide | wild type enzyme, in 5 mM Tris, 0.1% (w/v) PEG 8000, pH 8.0, 25°C, 200 mM NaCl | Homo sapiens | |
0.00088 | - |
D-Phe-Pro-Arg-4-nitroanilide | wild type enzyme, in 5 mM Tris, 0.1% (w/v) PEG 8000, pH 8.0, 25°C, 200 mM choline chloride | Homo sapiens | |
0.0029 | - |
D-Phe-Pro-Arg-4-nitroanilide | mutant enzyme N143P, in 5 mM Tris, 0.1% (w/v) PEG 8000, pH 8.0, 25°C, 200 mM NaCl | Homo sapiens | |
0.004 | - |
D-Phe-Pro-Lys-4-nitroanilide | wild type enzyme, in 5 mM Tris, 0.1% (w/v) PEG 8000, pH 8.0, 25°C, 200 mM NaCl | Homo sapiens | |
0.0064 | - |
D-Phe-Pro-Lys-4-nitroanilide | mutant enzyme N143P, in 5 mM Tris, 0.1% (w/v) PEG 8000, pH 8.0, 25°C, 200 mM NaCl | Homo sapiens | |
0.013 | - |
D-Phe-Pro-Arg-4-nitroanilide | mutant enzyme N143P, in 5 mM Tris, 0.1% (w/v) PEG 8000, pH 8.0, 25°C, 200 mM choline chloride | Homo sapiens | |
0.017 | - |
D-Phe-Pro-Phe-4-nitroanilide | wild type enzyme, in 5 mM Tris, 0.1% (w/v) PEG 8000, pH 8.0, 25°C, 200 mN NaCl | Homo sapiens | |
0.033 | - |
D-Phe-Pro-Phe-4-nitroanilide | mutant enzyme N143P, in 5 mM Tris, 0.1% (w/v) PEG 8000, pH 8.0, 25°C, 200 mN NaCl | Homo sapiens | |
0.04 | - |
D-Phe-Pro-Phe-4-nitroanilide | mutant enzyme N143P, in 5 mM Tris, 0.1% (w/v) PEG 8000, pH 8.0, 25°C, 200 mM choline chloride | Homo sapiens | |
0.04 | - |
D-Phe-Pro-Lys-4-nitroanilide | wild type enzyme, in 5 mM Tris, 0.1% (w/v) PEG 8000, pH 8.0, 25°C, 200 mM choline chloride | Homo sapiens | |
0.072 | - |
D-Phe-Pro-Lys-4-nitroanilide | mutant enzyme N143P, in 5 mM Tris, 0.1% (w/v) PEG 8000, pH 8.0, 25°C, 200 mM choline chloride | Homo sapiens | |
0.11 | - |
D-Phe-Pro-Phe-4-nitroanilide | wild type enzyme, in 5 mM Tris, 0.1% (w/v) PEG 8000, pH 8.0, 25°C, 200 mM choline chloride | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Na+ | Na+ activates thrombin by securing the correct orientation of the Glu192-Gly193 peptide bond, which is likely flipped in the absence of cation | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
fibrinogen + H2O | Homo sapiens | - |
fibrin + fibrinopeptide A + fibrinopeptide B | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Phe-Pro-Arg-4-nitroanilide + H2O | - |
Homo sapiens | D-Phe-Pro-Arg + 4-nitroaniline | - |
? | |
D-Phe-Pro-Lys-4-nitroanilide + H2O | - |
Homo sapiens | D-Phe-Pro-Lys + 4-nitroaniline | - |
? | |
D-Phe-Pro-Phe-4-nitroanilide + H2O | - |
Homo sapiens | D-Phe-Pro-Phe + 4-nitroaniline | - |
? | |
fibrinogen + H2O | - |
Homo sapiens | fibrin + fibrinopeptide A + fibrinopeptide B | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
D-Phe-Pro-Lys-4-nitroanilide | mutant enzyme N143P, in 5 mM Tris, 0.1% (w/v) PEG 8000, pH 8.0, 25°C, 200 mM NaCl | Homo sapiens | |
0.018 | - |
D-Phe-Pro-Phe-4-nitroanilide | mutant enzyme N143P, in 5 mM Tris, 0.1% (w/v) PEG 8000, pH 8.0, 25°C, 200 mN NaCl | Homo sapiens | |
0.23 | - |
D-Phe-Pro-Phe-4-nitroanilide | mutant enzyme N143P, in 5 mM Tris, 0.1% (w/v) PEG 8000, pH 8.0, 25°C, 200 mM choline chloride | Homo sapiens | |
0.26 | - |
D-Phe-Pro-Lys-4-nitroanilide | mutant enzyme N143P, in 5 mM Tris, 0.1% (w/v) PEG 8000, pH 8.0, 25°C, 200 mM choline chloride | Homo sapiens | |
1.4 | - |
D-Phe-Pro-Phe-4-nitroanilide | wild type enzyme, in 5 mM Tris, 0.1% (w/v) PEG 8000, pH 8.0, 25°C, 200 mM choline chloride | Homo sapiens | |
2 | 8 | D-Phe-Pro-Lys-4-nitroanilide | wild type enzyme, in 5 mM Tris, 0.1% (w/v) PEG 8000, pH 8.0, 25°C, 200 mM NaCl | Homo sapiens | |
3.5 | - |
D-Phe-Pro-Arg-4-nitroanilide | wild type enzyme, in 5 mM Tris, 0.1% (w/v) PEG 8000, pH 8.0, 25°C, 200 mM choline chloride | Homo sapiens | |
6 | - |
D-Phe-Pro-Arg-4-nitroanilide | mutant enzyme N143P, in 5 mM Tris, 0.1% (w/v) PEG 8000, pH 8.0, 25°C, 200 mM NaCl | Homo sapiens | |
7.5 | - |
D-Phe-Pro-Arg-4-nitroanilide | mutant enzyme N143P, in 5 mM Tris, 0.1% (w/v) PEG 8000, pH 8.0, 25°C, 200 mM choline chloride | Homo sapiens | |
8.3 | - |
D-Phe-Pro-Phe-4-nitroanilide | wild type enzyme, in 5 mM Tris, 0.1% (w/v) PEG 8000, pH 8.0, 25°C, 200 mN NaCl | Homo sapiens | |
12 | - |
D-Phe-Pro-Lys-4-nitroanilide | wild type enzyme, in 5 mM Tris, 0.1% (w/v) PEG 8000, pH 8.0, 25°C, 200 mM choline chloride | Homo sapiens | |
29 | - |
D-Phe-Pro-Arg-4-nitroanilide | wild type enzyme, in 5 mM Tris, 0.1% (w/v) PEG 8000, pH 8.0, 25°C, 200 mM NaCl | Homo sapiens |