Literature summary for 3.4.21.5 extracted from

Hu, L.; Ibrahim, S.; Liu, C.; Skaar, J.; Pagano, M.; Karpatkin, S.
Thrombin induces tumor cell cycle activation and spontaneous growth by down-regulation of p27Kip1, in association with the up-regulation of Skp2 and MiR-222 (2009), Cancer Res., 69, 3374-3382.

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Application

Application	Comment	Organism
medicine	examination of the effect of thrombin on tumor cell cycle activation and spontaneous growth in synchronized serum-starved tumor cell lines and a model of spontaneous prostate cancer development in TRAMP mice expressing the SV40 Large T Antigen under the control of the prostate-specific probasin promoter, leading to the spontaneous development of prostate cancer and metastasis. Prostate LNCaP cells arrested in G0 and treated with thrombin or serum reveal a 48- and 29-fold increase in S phase cells, respectively, at 8 hours. Addition of thrombin, PAR-1 agonist TFLLRN, or serum down-regulates the inhibitory cell cycle regulator p27Kip1 with concomitant induction of Skp2, cyclin D1, and cyclin A with similar kinetics. LNCaP p27Kip1-transfected cells or Skp2 knockdown cells are refractory to thrombin-induced cell cycle activation. Repetitive thrombin injection enhances prostate tumor volume 6- to 8-fold. Repetitive hirudin injection, a specific potent antithrombin, decreases tumor volume 13- to 24fold	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	commercial product	-

Source Tissue

Source Tissue	Comment	Organism	Textmining

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Release 2023.1 (January 2023)