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Literature summary for 3.4.21.5 extracted from

  • Radau, G.; Fokkens, J.
    Design and X-ray crystal structures of human thrombin with synthetic cyanopeptide-analogues (2007), Pharmazie, 62, 83-88.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
purified hirudin-alpha-thrombin in complex with inhibitors RA-1008, RA-1014, aeruginosin 98-B, and aeruginosin 298-A, hanging-drop vapour diffusion method, the protein solution contains 4-5 mg/ml protein, 50 mg/ml hirudin, and 2-3 mM inhibitor and is mixed with reservoir solution containing 22-27% polyethylene glycol 8000 and 0.1 M sodium phosphate buffer, pH 7.5, a few days to microcrystals, macroseeding to obtain laregr crystals, X-ray diffraction structure determination and analysis at 1.85 A resolution, modeling Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
aeruginosin 298-A isolated from Microcystis aeruginosa strain NIES-298 Homo sapiens
aeruginosin 98-B isolated from Microcystis aeruginosa strain NIES-98 Homo sapiens
RA-1008 a synthetic, low-molecular cyanopeptide-analogue inhibitor, binding structure and inhibition mechanism, overview Homo sapiens
RA-1014 a synthetic, low-molecular cyanopeptide-analogue inhibitor, binding structure and inhibition mechanism, overview Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation alpha-thrombin Homo sapiens
-

Synonyms

Synonyms Comment Organism
alpha-thrombin
-
Homo sapiens