Crystallization (Comment) | Organism |
---|---|
purified hirudin-alpha-thrombin in complex with inhibitors RA-1008, RA-1014, aeruginosin 98-B, and aeruginosin 298-A, hanging-drop vapour diffusion method, the protein solution contains 4-5 mg/ml protein, 50 mg/ml hirudin, and 2-3 mM inhibitor and is mixed with reservoir solution containing 22-27% polyethylene glycol 8000 and 0.1 M sodium phosphate buffer, pH 7.5, a few days to microcrystals, macroseeding to obtain laregr crystals, X-ray diffraction structure determination and analysis at 1.85 A resolution, modeling | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
aeruginosin 298-A | isolated from Microcystis aeruginosa strain NIES-298 | Homo sapiens | |
aeruginosin 98-B | isolated from Microcystis aeruginosa strain NIES-98 | Homo sapiens | |
RA-1008 | a synthetic, low-molecular cyanopeptide-analogue inhibitor, binding structure and inhibition mechanism, overview | Homo sapiens | |
RA-1014 | a synthetic, low-molecular cyanopeptide-analogue inhibitor, binding structure and inhibition mechanism, overview | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | alpha-thrombin | Homo sapiens | - |
Synonyms | Comment | Organism |
---|---|---|
alpha-thrombin | - |
Homo sapiens |