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Literature summary for 3.4.21.5 extracted from

  • Bush, L.A.; Nelson, R.W.; Di Cera, E.
    Murine thrombin lacks Na+ activation but retains high catalytic activity (2006), J. Biol. Chem., 281, 7183-7188.
    View publication on PubMed
Search for more literature for 3.4.21.5

Activating Compound

Activating Compound Comment Organism Structure
additional information not activating: Na+. High activity even in absence of Na+ due to K222D change in amino acids compared to human enzyme Mus musculus
Na+ increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+ Homo sapiens
Na+ not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+ Mus musculus

Protein Variants

Protein Variants Comment Organism
K222D introduction of activation by Na+ similar to human enzyme, wild-type is not activated by Na+ Mus musculus
W215A/E217A largely compromised fibrinogen cleavage, severe reduction in cleavage of PAR1, PAR4 Mus musculus
W215A/E217A strikingly compromised fibrinogen cleavage, severe reduction in cleavage of PAR1, PAR4 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information ratios of kcat/KM for substrates fibrinogen, PAR1, PAR4, protein C and RAP and comparison with human enzyme Mus musculus
additional information
-
 additional information ratios of kcat/KM for substrates fibrinogen, PAR1, PAR4, protein C and RAP and comparison with human enzyme Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Mus musculus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Phe-L-Pro-L-Arg-4-nitroanilide + H2O
-
 Mus musculus D-Phe-L-Pro-L-Arg + 4-nitroaniline
-
 ?
D-Phe-L-Pro-L-Arg-4-nitroanilide + H2O
-
 Homo sapiens D-Phe-L-Pro-L-Arg + 4-nitroaniline
-
 ?
D-Phe-L-Pro-L-Phe-4-nitroanilide + H2O
-
 Mus musculus D-Phe-L-Pro-L-Phe + 4-nitroaniline
-
 ?
D-Phe-L-Pro-L-Phe-4-nitroanilide + H2O
-
 Homo sapiens D-Phe-L-Pro-L-Phe + 4-nitroaniline
-
 ?
fibrinogen + H2O
-
 Homo sapiens fibrin + fibrinopeptide A + fibrinopeptide B
-
 ?
PAR1 + H2O
-
 Homo sapiens ?
-
 ?
PAR4 + H2O
-
 Homo sapiens ?
-
 ?
protein C + H2O
-
 Homo sapiens ?
-
 ?
RAP + H2O
-
 Homo sapiens ?
-
 ?
spectrozyme-TH + H2O
-
 Mus musculus ?
-
 ?
spectrozyme-TH + H2O
-
 Homo sapiens ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
15
-
 spectrozyme-TH pH 8.0, 25°C, absence of Na+ Homo sapiens
21
-
 spectrozyme-TH mutant K222D, pH 8.0, 25°C, absence of Na+ Mus musculus
32
-
 spectrozyme-TH wild-type, pH 8.0, 25°C, absence of Na+ Mus musculus
32
-
 spectrozyme-TH wild-type, pH 8.0, 25°C, presence of 0.2 M Na+ Mus musculus
80
-
 spectrozyme-TH mutant K222D, pH 8.0, 25°C, presence of 0.2 M Na+ Mus musculus
91
-
 spectrozyme-TH pH 8.0, 25°C, presence of 0.2 M Na+ Homo sapiens