Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | not activating: Na+. High activity even in absence of Na+ due to K222D change in amino acids compared to human enzyme | Mus musculus | |
Na+ | increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+ | Homo sapiens | |
Na+ | not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+ | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
K222D | introduction of activation by Na+ similar to human enzyme, wild-type is not activated by Na+ | Mus musculus |
W215A/E217A | largely compromised fibrinogen cleavage, severe reduction in cleavage of PAR1, PAR4 | Mus musculus |
W215A/E217A | strikingly compromised fibrinogen cleavage, severe reduction in cleavage of PAR1, PAR4 | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | ratios of kcat/KM for substrates fibrinogen, PAR1, PAR4, protein C and RAP and comparison with human enzyme | Mus musculus | |
additional information | - |
additional information | ratios of kcat/KM for substrates fibrinogen, PAR1, PAR4, protein C and RAP and comparison with human enzyme | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Mus musculus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Phe-L-Pro-L-Arg-4-nitroanilide + H2O | - |
Mus musculus | D-Phe-L-Pro-L-Arg + 4-nitroaniline | - |
? | |
D-Phe-L-Pro-L-Arg-4-nitroanilide + H2O | - |
Homo sapiens | D-Phe-L-Pro-L-Arg + 4-nitroaniline | - |
? | |
D-Phe-L-Pro-L-Phe-4-nitroanilide + H2O | - |
Mus musculus | D-Phe-L-Pro-L-Phe + 4-nitroaniline | - |
? | |
D-Phe-L-Pro-L-Phe-4-nitroanilide + H2O | - |
Homo sapiens | D-Phe-L-Pro-L-Phe + 4-nitroaniline | - |
? | |
fibrinogen + H2O | - |
Homo sapiens | fibrin + fibrinopeptide A + fibrinopeptide B | - |
? | |
PAR1 + H2O | - |
Homo sapiens | ? | - |
? | |
PAR4 + H2O | - |
Homo sapiens | ? | - |
? | |
protein C + H2O | - |
Homo sapiens | ? | - |
? | |
RAP + H2O | - |
Homo sapiens | ? | - |
? | |
spectrozyme-TH + H2O | - |
Mus musculus | ? | - |
? | |
spectrozyme-TH + H2O | - |
Homo sapiens | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15 | - |
spectrozyme-TH | pH 8.0, 25°C, absence of Na+ | Homo sapiens | |
21 | - |
spectrozyme-TH | mutant K222D, pH 8.0, 25°C, absence of Na+ | Mus musculus | |
32 | - |
spectrozyme-TH | wild-type, pH 8.0, 25°C, absence of Na+ | Mus musculus | |
32 | - |
spectrozyme-TH | wild-type, pH 8.0, 25°C, presence of 0.2 M Na+ | Mus musculus | |
80 | - |
spectrozyme-TH | mutant K222D, pH 8.0, 25°C, presence of 0.2 M Na+ | Mus musculus | |
91 | - |
spectrozyme-TH | pH 8.0, 25°C, presence of 0.2 M Na+ | Homo sapiens |