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Literature summary for 3.4.21.5 extracted from

  • Michaud, S.E.; Wang, L.Z.; Korde, N.; Bucki, R.; Randhawa, P.K.; Pastore, J.J.; Falet, H.; Hoffmeister, K.; Kuuse, R.; Uibo, R.; Herod, J.; Sawyer, E.; Janmey, P.A.
    Purification of salmon thrombin and its potential as an alternative to mammalian thrombins in fibrin sealants (2002), Thromb. Res., 107, 245-254.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ slight inhibition at 10 mM Homo sapiens
Ca2+ 50% inhibition at 10 mM Salmo salar

Metals/Ions

Metals/Ions Comment Organism Structure
additional information not affected by NaCl up to 600 mM Homo sapiens
NaCl 50% activation at 600 mM Salmo salar

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens enzyme stimulates platelets and exposure of phosphatidylserine on the external surface ?
-
?
additional information Salmo salar enzyme stimulates platelets and exposure of phosphatidylserine on the external surface ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Salmo salar
-
salmon
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification activation of prothrombin by snake venom from Oxyuranus scutellatus Salmo salar

Purification (Commentary)

Purification (Comment) Organism
from plasma, to homogeneity Salmo salar

Source Tissue

Source Tissue Comment Organism Textmining
plasma
-
Salmo salar
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Salmo salar

Storage Stability

Storage Stability Organism
0°C, on ice, 8 h, stable Salmo salar
22°C, at room temperature, several hours, stable, lyophilized stable for several months Salmo salar

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
fibrinogen + H2O from human and salmon Salmo salar fibrin + fibrinopeptide A + fibrinopeptide B
-
?
additional information enzyme stimulates platelets and exposure of phosphatidylserine on the external surface Homo sapiens ?
-
?
additional information enzyme stimulates platelets and exposure of phosphatidylserine on the external surface Salmo salar ?
-
?
tosyl-Gly-Pro-Arg-4-nitroanilide + H2O
-
Homo sapiens tosyl-Gly-Pro-Arg + 4-nitroaniline
-
?
tosyl-Gly-Pro-Arg-4-nitroanilide + H2O
-
Salmo salar tosyl-Gly-Pro-Arg + 4-nitroaniline
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Salmo salar
35
-
-
Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
lyophilized enzyme, 8 h, 90% remaining activity Homo sapiens
80
-
lyophilized purified enzyme, 8 h, 80% remaining activity Salmo salar

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Homo sapiens
8.5
-
-
Salmo salar