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Literature summary for 3.4.21.5 extracted from
- Interaction of the factor XIII activation peptide with alpha-thrombin. Crystal structure of its enzyme-substrate analog complex (2000), J. Biol. Chem., 275, 36942-36948.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| 8 mg/ml purified enzyme in complex with factor XIII(28-37) peptide as a chloromethylketone derivative, hanging drop vapor diffusion method, room temperature, 24% PEG 8000, 200 mM sodium chloride, 50 mM sodium citrate, pH 5.5, 2 weeks for full size crystals, X-ray diffraction structure determination and analysis at 1.6 A | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pro-factor XIII + H2O | Homo sapiens | activation by cleavage at Arg37 leading to blood coagulation | factor XIII | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| selective cleavage of Arg-/-Gly bonds in fibrinogen to form fibrin and release fibrinopeptides A and B | serine protease, for substrate binding P1 and P2 residues must be compatible with the geometry and chemistry of S1 and S2 specificity sites in thrombin, a glycine in P5 position is necessary for conserved substrate conformation, the hydrophobic residues, which occupy the aryl binding site of the enzyme determine the substrate conformation further away from the catalytic residues | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| fibrinopeptide A + H2O | - |
Homo sapiens | ? | - |
? | |
| pro-factor XIII + H2O | activation by cleavage at Arg37 leading to blood coagulation | Homo sapiens | factor XIII | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-thrombin | - |
Homo sapiens |
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