Cloned (Comment) | Organism |
---|---|
expression of C2 in insect cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
Mg2+-bound C2a, hanging drop vapor diffusion method, 22°C, 0.002 ml of 20 mg/ml protein solution is mixed with 0.002 ml of well solution containing 20% w/v polyethylene glycol 10000, 0.1 M HEPES, pH 7.5, and 0.0004 ml of 0.3 M glycyl-glycyl-glycine, 3-7 days, X-ray diffraction structure determination and analysis at 1.9-2.3 A resolution | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required during enzyme formation | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | the multi-domain serine protease C2 is the catalytic fragment of classical pathway C3 and C5 convertase of human complement of the classical and lectin pathways of complement activation, formation of these convertases requires the Mg2+-dependent binding of C2 to C4b and the subsequent cleavage of C2 by C1s or MASP2, respectively, the C-terminal fragment C2a consisting of a serine protease and a von Willebrand factor type A domain, remains attached to C4b, forming the C3 convertase, C4b2a, structure, overview | ? | - |
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Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | C2 is a glycosylated modular protein consisting of three domains | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the multi-domain serine protease C2 is the catalytic fragment of classical pathway C3 and C5 convertase of human complement of the classical and lectin pathways of complement activation, formation of these convertases requires the Mg2+-dependent binding of C2 to C4b and the subsequent cleavage of C2 by C1s or MASP2, respectively, the C-terminal fragment C2a consisting of a serine protease and a von Willebrand factor type A domain, remains attached to C4b, forming the C3 convertase, C4b2a, structure, overview | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | C2 is a glycosylated modular protein consisting of three domains, C2a is the catalytic fragment of classical pathway C3 and C5 convertase of human complement, formation of the convertases requires the Mg2+-dependent binding of C2 to C4b and the subsequent cleavage of C2 by C1s or MASP2, respectively, the C-terminal fragment C2a consisting of a serine protease and a von Willebrand factor type A domain, remains attached to C4b, forming the C3 convertase, C4b2a, three-dimensional structure of the C2a molecule, residues Asp240, Ser242, Ser244, Thr317 and Asp356 constitute the MIDAS motif of C2a, overview | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
C3 convertase | - |
Homo sapiens |
C3bBb | alternative pathway C3 convertase | Homo sapiens |
C4b2a | - |
Homo sapiens |
C5 convertase | - |
Homo sapiens |