Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Aprotinin | blocks increase of DU145 cell migration and invasion by tissue kallikrein | Rattus norvegicus | |
icatibant | minimally affects increase of DU145 cell migration and invasion by tissue kallikrein | Rattus norvegicus | |
additional information | kinin has little effect on increase of DU145 cell migration and invasion by tissue kallikrein. Tissue kallikrein-induced cell migration and invasion are abolished by inhibition of PAR1 using a pharmacological inhibitor or RNA interference. The effect of tissue kallikrein on cell migration and invasion are also blocked by inhibitors of protein kinase C, c-Src, matrix metalloproteinase, EGFR and extracellular signal-regulated kinase | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
x * 35000, SDS-PAGE | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by affinity column chromatography | Homo sapiens |
by affinity column chromatography | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
salivary gland | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Val-Leu-Arg-4-nitroanilide + H2O | - |
Rattus norvegicus | D-Val-Leu-Arg + 4-nitroaniline | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 35000, SDS-PAGE | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
tissue kallikrein | - |
Homo sapiens |
tissue kallikrein | - |
Rattus norvegicus |
General Information | Comment | Organism |
---|---|---|
physiological function | purified tissue kallikrein promotes DU145 prostate cancer cell migration in a similar manner than rat tissue kallikrein | Homo sapiens |
physiological function | tissue kallikrein promotes DU145 prostate cancer cell migration in a concentration-dependent manner, but has no effect on A549 lung cancer cells. Tissue kallikrein stimulates DU145 cell proliferation through activation of the kinin B2 receptor, but not PAR1 and EGFR activation. Tissue kallikrein promotes migration and invasion of DU145 cells through PAR1-PKC-Src-MMP activation and EGFR-ERK transactivation. Tissue kallikrein stimulates ERK activation through EGFR transactivation. Differential signaling pathways mediated by tissue kallikrein in promoting prostate cancer cell migration and invasion via PAR1 activation, and proliferation via kinin B2 receptor stimulation. Stimulates ERK phosphorylation | Rattus norvegicus |