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Literature summary for 3.4.21.35 extracted from

  • Chao, J.; Chao, L.
    Rat urinary kallikrein (1988), Methods Enzymol., 163, 128-143.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
bovine serum albumin 1 mg/ml, activates Rattus norvegicus
Brij-58 0.1%, activates Rattus norvegicus
deoxycholate 0.1%, activates Rattus norvegicus
Lima-bean trypsin inhibitor 0.017 mg/ml, activates Rattus norvegicus
Lubrol Px 0.1%, activates Rattus norvegicus
ovomucoid trypsin inhibitor 0.017 mg/ml, activates Rattus norvegicus
Triton X-100 0.1%, activates Rattus norvegicus

General Stability

General Stability Organism
freezing and thawing results in at least three-fold increase of specific activity Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
amiloride
-
Rattus norvegicus
Aprotinin
-
Rattus norvegicus
benzamidine
-
Rattus norvegicus
diisopropyl fluorophosphate at a slow rate Rattus norvegicus
kallikrein antibody
-
Rattus norvegicus
leupeptin
-
Rattus norvegicus
Pentamidine
-
Rattus norvegicus
Phe-Phe-Arg-CH2Cl
-
Rattus norvegicus
Soybean trypsin inhibitor
-
Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular secretion Rattus norvegicus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
polypeptide + H2O Rattus norvegicus
-
peptides
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification urinary and renal kallikrein exist in both active and proenzyme form Rattus norvegicus
proteolytic modification activation by trypsin Rattus norvegicus
side-chain modification multiple forms are due at least in part to varying carbohydrates Rattus norvegicus

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
Rattus norvegicus
-
urine
-
Rattus norvegicus
-

Storage Stability

Storage Stability Organism
-20°C, 0.1 N NaCl, 0.01 M sodium phosphate buffer, pH 7.0, stable for months Rattus norvegicus
4°C, stable for weeks Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Val-Leu-Arg-p-nitroanilide + H2O
-
Rattus norvegicus D-Val-Leu-Arg + p-nitroaniline
-
?
kininogen + H2O high molecular weight kininogen from human and rat Rattus norvegicus kinin + ?
-
?
kininogen + H2O cleavage of rat kininogen at Arg-Arg and Arg-Ser Rattus norvegicus bradykinin + kinin heavy chain + kinin light chain
-
?
low-molecular-weight kininogen + H2O bovine or human Rattus norvegicus kallidin + kinin
-
?
polypeptide + H2O
-
Rattus norvegicus peptides
-
?
Tosyl-Arg methyl ester + H2O
-
Rattus norvegicus Tosyl-Arg + methanol
-
?

Subunits

Subunits Comment Organism
monomer 1 * 38000, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
18 h, 70% of the activity is retained Rattus norvegicus
100
-
5 min, 60% loss of activity Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Rattus norvegicus