Activating Compound | Comment | Organism | Structure |
---|---|---|---|
bovine serum albumin | 1 mg/ml, activates | Rattus norvegicus | |
Brij-58 | 0.1%, activates | Rattus norvegicus | |
deoxycholate | 0.1%, activates | Rattus norvegicus | |
Lima-bean trypsin inhibitor | 0.017 mg/ml, activates | Rattus norvegicus | |
Lubrol Px | 0.1%, activates | Rattus norvegicus | |
ovomucoid trypsin inhibitor | 0.017 mg/ml, activates | Rattus norvegicus | |
Triton X-100 | 0.1%, activates | Rattus norvegicus |
General Stability | Organism |
---|---|
freezing and thawing results in at least three-fold increase of specific activity | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
amiloride | - |
Rattus norvegicus | |
Aprotinin | - |
Rattus norvegicus | |
benzamidine | - |
Rattus norvegicus | |
diisopropyl fluorophosphate | at a slow rate | Rattus norvegicus | |
kallikrein antibody | - |
Rattus norvegicus | |
leupeptin | - |
Rattus norvegicus | |
Pentamidine | - |
Rattus norvegicus | |
Phe-Phe-Arg-CH2Cl | - |
Rattus norvegicus | |
Soybean trypsin inhibitor | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | secretion | Rattus norvegicus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
polypeptide + H2O | Rattus norvegicus | - |
peptides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | urinary and renal kallikrein exist in both active and proenzyme form | Rattus norvegicus |
proteolytic modification | activation by trypsin | Rattus norvegicus |
side-chain modification | multiple forms are due at least in part to varying carbohydrates | Rattus norvegicus |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Rattus norvegicus | - |
urine | - |
Rattus norvegicus | - |
Storage Stability | Organism |
---|---|
-20°C, 0.1 N NaCl, 0.01 M sodium phosphate buffer, pH 7.0, stable for months | Rattus norvegicus |
4°C, stable for weeks | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Val-Leu-Arg-p-nitroanilide + H2O | - |
Rattus norvegicus | D-Val-Leu-Arg + p-nitroaniline | - |
? | |
kininogen + H2O | high molecular weight kininogen from human and rat | Rattus norvegicus | kinin + ? | - |
? | |
kininogen + H2O | cleavage of rat kininogen at Arg-Arg and Arg-Ser | Rattus norvegicus | bradykinin + kinin heavy chain + kinin light chain | - |
? | |
low-molecular-weight kininogen + H2O | bovine or human | Rattus norvegicus | kallidin + kinin | - |
? | |
polypeptide + H2O | - |
Rattus norvegicus | peptides | - |
? | |
Tosyl-Arg methyl ester + H2O | - |
Rattus norvegicus | Tosyl-Arg + methanol | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 38000, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
18 h, 70% of the activity is retained | Rattus norvegicus |
100 | - |
5 min, 60% loss of activity | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Rattus norvegicus |