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Literature summary for 3.4.21.35 extracted from
- Rat pancreas kallikrein (1988), Methods Enzymol., 163, 115-128.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35900 | - |
prokallikrein A, gel filtration | Rattus norvegicus |
| 36500 | - |
1 * 36500, prokallikrein A, SDS-PAGE | Rattus norvegicus |
| 37400 | - |
prokallikrein B, gel filtration | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| polypeptide + H2O | Rattus norvegicus | - |
peptides | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | zymogen: prokallikrein | Rattus norvegicus |
| proteolytic modification | activation by trypsin | Rattus norvegicus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| prokallikrein A and B | Rattus norvegicus |
| to homogeneity | Rattus norvegicus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 315 | - |
purified kallikrein A | Rattus norvegicus |
| 323 | - |
purified kallikrein B | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzyloxycarbonyl-Arg methyl ester + H2O | - |
Rattus norvegicus | benzyloxycarbonyl-Arg + methanol | - |
? | |
| Benzyloxycarbonyl-Lys methyl ester + H2O | - |
Rattus norvegicus | Benzyloxycarbonyl-Lys + methanol | - |
? | |
| N-alpha-acetyl-Tyr ethyl ester + H2O | - |
Rattus norvegicus | N-alpha-acetyl-Tyr + ethanol | - |
? | |
| N-alpha-benzoyl-L-Arg ethyl ester + H2O | - |
Rattus norvegicus | N-alpha-benzoyl-L-Arg + ethanol | - |
? | |
| polypeptide + H2O | - |
Rattus norvegicus | peptides | - |
? | |
| Tosyl-Arg methyl ester + H2O | - |
Rattus norvegicus | Tosyl-Arg + methanol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 36500, prokallikrein A, SDS-PAGE | Rattus norvegicus |
| monomer | 1* 38400, prokallikrein B, SDS-PAGE | Rattus norvegicus |
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