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Literature summary for 3.4.21.35 extracted from

  • Takada, Y.; Skidgel, R.A.; Erdös, E.G.
    Purification of human urinary prokallikrein (1985), Biochem. J., 232, 851-858.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
Thermolysin thermolysin activates prokallikrein cleaving off the N-terminal Ile1-Val2 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular secretion Homo sapiens
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
1 * 46000, active kallikrein, SDS-PAGE Homo sapiens
48000
-
1 * 48000, prokallikrein, SDS-PAGE Homo sapiens
53000
-
prokallikrein and kallikrein, gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
polypeptide + H2O Homo sapiens
-
peptides
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification thermolysin activates prokallikrein cleaving off the N-terminal Ile1-Val2 Homo sapiens
proteolytic modification zymogen: prokallikrein can by activated by two types of enzymes, serine proteases which cleave at the C-terminus of basic amino acids and by a metalloproteinase that cleaves at the N-terminus of hydrophobic amino acids Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
prokallikrein, 183fold Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
urine
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
47
-
purified enzyme Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Val-Leu-Arg-7-amido-4-trifluoromethylcoumarin + H2O
-
Homo sapiens D-Val-Leu-Arg + 7-amino-4-trifluoromethylcoumarin
-
?
polypeptide + H2O
-
Homo sapiens peptides
-
?

Subunits

Subunits Comment Organism
monomer 1 * 48000, prokallikrein, SDS-PAGE Homo sapiens
monomer 1 * 46000, active kallikrein, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Homo sapiens