Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Thermolysin | thermolysin activates prokallikrein cleaving off the N-terminal Ile1-Val2 | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | secretion | Homo sapiens | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
1 * 46000, active kallikrein, SDS-PAGE | Homo sapiens |
48000 | - |
1 * 48000, prokallikrein, SDS-PAGE | Homo sapiens |
53000 | - |
prokallikrein and kallikrein, gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
polypeptide + H2O | Homo sapiens | - |
peptides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | thermolysin activates prokallikrein cleaving off the N-terminal Ile1-Val2 | Homo sapiens |
proteolytic modification | zymogen: prokallikrein can by activated by two types of enzymes, serine proteases which cleave at the C-terminus of basic amino acids and by a metalloproteinase that cleaves at the N-terminus of hydrophobic amino acids | Homo sapiens |
Purification (Comment) | Organism |
---|---|
prokallikrein, 183fold | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
urine | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
47 | - |
purified enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Val-Leu-Arg-7-amido-4-trifluoromethylcoumarin + H2O | - |
Homo sapiens | D-Val-Leu-Arg + 7-amino-4-trifluoromethylcoumarin | - |
? | |
polypeptide + H2O | - |
Homo sapiens | peptides | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 48000, prokallikrein, SDS-PAGE | Homo sapiens |
monomer | 1 * 46000, active kallikrein, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |